视网膜色素上皮、Bruch膜及脉络膜老年性改变的定量分析

目的 研究及定量分析视网膜色素上皮（retinal pigment epithelium，RPE)、Bruch膜及脉络膜的老年性改变。方法 应用改良Masson三色组织染色法及电脑图像分析软件对36例不同年龄(3～93岁)正常眼RPE数量和高度、Bruch膜厚度、脉络膜毛细血管的密度及脉络膜厚度的老年性改变进行定量分析。 结果 与年龄有显著相关性的老年性改变包括RPE细胞数量的减少(8个/ mm²·年)、RPE细胞高度的增加(0.01μm/年) 以及Bruch膜增厚(0.02μm/年)；脉络膜毛细血管密度及脉络膜厚度的改变与年龄之间无显著的相关关系。 结论 RPE细胞数量减少、高度增加以及Bruch膜的增厚可能是黄斑区最早的老年性改变。(中华眼底病杂志,2000,16:236-239)

A morphometric analysis of age-related changes in the RPE, Bruch's membrane and choroid of human eyes

Purpose To identify and quantitatively evaluate age-related changes in the retinal pigment epithelium (RPE) and underlying Bruch is membrane and choroid in donor human eyes. Methods 36unpaired human eyes of varying age (3-39 years) from Caucasian donors were supplied by Manchester Eye Bank (UK) or National Disease Research Interchange (Philadephia,USA).Modified Masson is trichrome staining was used to illustrate age-related changes in RPE cell, Bruch is membrane thickness, and density of choriocapillaries and thickness of the choroid. Data were assessed using computer-aided quantitative morphometric analysis method. ResultsThe thickness of Bruch is membrane increased with age while there is a change in morphology of RPE cells including a decrease in number and RPE cell thickening with age. RPE cells decreased at a rate of 8 cells/mm² · year, RPE cell height and thickness of Bruch is membrane increased at rates of 0.01(μm/year) and 0.02 (μm/year) respectively. The luminal area of choriocapillaries and the thickness of choroid showed no close relation with age. Conclusion RPE cell loss and thickening of Bruch is membrane and RPE cells may be the earlier and primary alteration with age. (Chin J Ocul Fundus Dis,2000,16:236-239)