神经营养因子NGF、BDNF对鼠胚基底前脑胆碱能神经元发育生长的协同作用

目的：探讨神经营养因子ＮＧＦ、ＢＤＮＦ联合使用时对鼠胚基义离胆碱能神经元发育生长的协同作用。方法：实验分成ＮＧＦ、ＢＤＮＦ、ＮＧＦ＋ＢＤＮＦ和对照４组。采用细胞培养和脑内移植手段及ＡＣｈＥ组化染色、显微测量和图象分析等技术。检测各组胚基底前脑培养４天和８天时的ＡＣｈＥ阳性神经元数、胞体直径、胞体发出的突直数及最长突起长度以及胚基底前脑移植至脑内４．５个月时移植区内ＡＣｈＥ阳性元数、细胞面积和纤维密

SYNERGIC EFFECTS OF NEUROTROPHINS NGF AND BDNF ON THE DEVELOPMENT OF THE FETAL FOREBRAIN CHOLINERGIC NEURONS IN VITRO AND IN VIVO

Objective:In the present study, the combination of two neurotrophic factors, i.e., NGF and BDNF were used to investigate the synergitic effects of these factors on the development of fetal forebrain cholinergic neurons. Methods: The experiments were divided into NGF, BDNF, NGF BDNF and control groups. Using the techniques of cell culture, brain transplantation, AChE histochemical staining, micromeasure and image analysis, the number of AChE positive neurons of fetal forebrain, the diameter of cell bodies, the number of processes and the length of the longest processes were detected respectively on the 4th and 8th day after culture and the number of AChE positive neurons, the size of neurons and the density of fibers in the grafts were also investigated 4.5 months after grafting.Results: In the cell culture, NGF mainly increased the number of AChE positive neurons and slightly promoted the development of the processes, while BDNF strongly stimulated the development of the cell bodies and processes. On 4 day after cell culture, the data of NGF BDNF group were superior to that of NGF group, but no difference between BDNF NGF group and NGF group. On the 8th day, the data of NGF BDNF group were better than that of NGF or BDNF group. In brain grafts, the morphological data using neurotrophic factors were superior to that of control group, while data of NGF BDNF group were better than NGF or BDNF group.Conclusion: The effective time of BDNF were earlier than that of NGF for the development of cholinergic neurons in embryonic basal forebrain in vitro. The effects of NGF and BDNF had its own strong points and combined use of NGF and BDNF may play the synergic role in vivo and vitro during the earlier development of the cholinergic neurons in embryonic basal forebrain.[