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Ⅱ型ZTC1+1天然澄清剂与大孔树脂联用在香菇多糖纯化中的作用
引用本文:王世宇,廖婉,付超美. Ⅱ型ZTC1+1天然澄清剂与大孔树脂联用在香菇多糖纯化中的作用[J]. 中国药房, 2007, 18(18): 1381-1383
作者姓名:王世宇  廖婉  付超美
作者单位:成都中医药大学药学院,成都市,611730
摘    要:目的:考察Ⅱ型ZTC1+1天然澄清剂与大孔吸附树脂联用在香菇多糖提取中的纯化效果,确定理想的纯化工艺。方法:用Ⅱ型ZTC1+1天然澄清剂代替醇沉工艺,并与大孔吸附树脂联用;采用蒽酮-硫酸法对香菇多糖含量进行测定。结果:采用Ⅱ型ZTC1+1天然澄清剂与大孔吸附树脂联用能显著提高香菇多糖的得率。结论:Ⅱ型ZTC1+1天然澄清剂与大孔吸树脂联用既可提高产品内在质量,又能缩短生产周期、减少有机溶剂的使用、降低成本,值得推广。

关 键 词:天然澄清剂  大孔吸附树脂  纯化  香菇多糖  含量
文章编号:1001-0408(2007)18-1381-03
收稿时间:2006-10-10
修稿时间:2006-10-102007-03-10

ZTC1 + 1 Ⅱ Natural Clarifying Agent Combined with Macroporous Resin in the Purification of Lentinan
WANG Shiyu,LIAO Wan,FU Chaomei. ZTC1 + 1 Ⅱ Natural Clarifying Agent Combined with Macroporous Resin in the Purification of Lentinan[J]. China Pharmacy, 2007, 18(18): 1381-1383
Authors:WANG Shiyu  LIAO Wan  FU Chaomei
Affiliation:Chengdu University of Traditional Chinese Medicine, Chengdu, 611730 China
Abstract:OBJECTIVE: To study the purification effect of ZTC1+ 1 Ⅱ natural clarifying agent combined with macroporous resin on the extraction of Lentinan and to determine the optimal purification process. METHODS: The ZTC1+ 1 Ⅱ natural clarifying agent was used to substitute alcohol precipitation technique and which was used concomitantly with macroporous resin in the purification of Lentinan. The content of Lentinan was determined by Anthrone-Sulphuric Acid method. RESULTS: ZTC1+ 1 Ⅱ natural clarifying agent and macroporous resin used in combination could significantly enhance the extraction yield of Lentinan.CONCLUSION: ZTC1+ 1 Ⅱ natural clarifying agent and macroporous resin used in combination can improve the internal quality of the product as well as shorten the production cycle, lessen the use of organic solvent and lower the cost, thus deserving to be popularized.
Keywords:Natural clarifying agent   Macroporous resin   Purification   Lentinan   Contents
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