HPLC法测定市售贝母中贝母乙素含量

目的：为了选择贝母乙素含量较高的贝母原材料，方法：建立了ＨＰＬＣ法分析贝母原材料中贝母乙素含量，贝母生药粉用氨水－９５％乙醇（１：５）混合液取，柱前用２，４－二硝基苯肼（ＤＮＰＨ）衍生，衍生化条件５０℃下反应３０min。分析色谱柱：ＨyopersilC18ODS2流动相；ＣＨ３ＣＮ－１００mmol/LCH3COONH4,(41:59)pH=5.0),流速为１．２mL/min,UV375nm检测.结果:４种市售贝母东贝母的乙素含量最高,结论;方法灵敏,重现性好,样品用量少,便于应用.

Quantitative determination of peiminine in Bulbus Fritillaria available on market by HPLC

Object To develop a method for the quantitative determination ofpeiminine in bullus Fritillariae as a guide for the choice of a better product on the market. Methods An HPLC method was developed. The powdered drug was extracted with a mixed solution of ammonia-95% alcohol (1:5). The crude peiminine obtained was derivatized to its 2,4-dinitro hydrazone at pH 2.0 and 50 ℃ for 30 min. The HPLC conditions were:Hypersil C18 column, with CH3CN:100 m Mol ammonium acetate (49:51) at pH 5.0 as the mobile phase and detected at the wave length of 375 nm. Results Among the 4 samples obtained from the market, F. thunbergii var. chekiangensis Hsiao et K. C. Hsia gave the highest peiminine content. Conclusion The method was highly sensitive with good reproducibility and need only a small amount of sample, suitable for practical use.