兔视网膜细胞问水肿及玻璃体收缩性状改变的相关研究

目的探讨玻璃体收缩与视网膜细胞问水肿的相关性。方法色素性兔17只17只眼，激光光凝诱发视网膜静脉阻塞(RVO)后1个月，视网膜血管分布区域作病理观察，对出现视网膜细胞间水肿者，测定其玻璃体内凝胶比重及重力下伸展度的改变，并通过II型胶原蛋白免疫印迹分析其相关的分子学机制。结果RVO后1个月，13只实验眼视网膜出现细胞问水肿，占76．5％；玻璃体出现凝缩、液化，收缩性增强；玻璃体内II型胶原的a单链相互交连成大分子的p和7重链结构，破坏了胶原支架的稳定性。结论视网膜细胞间水肿与玻璃体收缩有相关性，玻璃体胶原的相互交连并导致支架稳定性的破坏是其主要原因。 （中华眼底病杂志，2004，20：29-32）

Relationship between retinal extracellular edema and vitreous contraction in rabbits

Objective To explore the correlation between retinal extracellular edema and vitreous contraction in rabbits. Methods Seventeen pigmented rabbit models with retinal vein occlusion (RVO)was set up by laser photocoagulation. Retinal vascularity area was pathologically examined 1 month later.The vitreous gellength under the gravity condition and the percentage of its weight in the rabbits with extraeellular edema was observed. The mechanisms were investigated by Western immunoblotting of type II collagen.Results Extracellular edema was found in 13 experimental eyes 1 month after the formation of RVO (76.5~) with contracted vitreous gel and released watery liquid, and the a component of type II collagen was cross-linked together to form high-molecular-weight components of 1] and 7, which weakened the stability of collagen net structure.Conclusions Vitreous contraction and retinal extracellular edemawere correlated. The main reason may be the cross-links of vitreous collagen that damages the stability of collagen structure. (Chin J Ocul Fundus Dis，2004，20：2-32)