重组人纤维蛋白片段包被培养对CIK增殖和活性的影响

【摘要】目的 重组人纤维蛋白片段（RetroNectin）包被培养对细胞因子诱导的杀伤细胞（cytokine-induced killer cells，CIK）体外增殖和杀伤活性的影响，以期获得扩增效率和活性更为理想的免疫活性细胞，为临床治疗提供依据。方法 提取急性白血病(Acute leukemia，AL)患者外周血单个核细胞，分别采用RetroNectin联合抗CD3单抗包被与传统方法培养获得CIK细胞，检测各组免疫活性细胞的增殖效率、上清液细胞因子含量、对自体白血病细胞的杀伤活性以及增殖过程中的凋亡情况。结果 ①不同RetroNectin浓度组CIK扩增倍数依次为254.31±41.03倍、233.35±50.12倍、174.29±32.83倍；传统方法CIK培养组为102.01±31.27（P<0.05）。②包被组培养上清液IL-2、IL-12、TNF、IFN-γ分泌高于传统培养方法组（P<0.05）。③CIK对自体白血病细胞的杀伤活性，RetroNectin包被组亦高于传统方法培养组（P<0.05）。④RetroNectin包被培养过程中细胞凋亡率较传统培养方法下降（P<0.05）。⑤细胞增殖、杀伤活性12.5ug/mL、9.375ug/mL的RetroNectin包被组均高于6.25ug/mL组（P<0.05），且前两组差异无统计学意义（P＞0.05）。结论 RetroNectin包被技术应用于CIK体外培养可以获得增殖率和活性更高的免疫活性细胞，是一种值得临床推荐的有效的培养方法。

Effect of RetroNectin on the Proliferation and Cellular Activity of Cytokine-Induced Killer Cells in Acute Leukemia

Objective:To investigate the effect of retronectin on cytokine-induced killer cells(CIKs) and the cytotoxicity to acute leukemia cells.Methods:Peripheral blood mononuclear cells(PBMC) were collected from 17 patients with acute leukemia,and divided into two groups:retronectin group and control group.Cells of retronectin group were seeded into culture flask precoated with retronectin and CD3mAb to induce CIKs,while cells of control group were seeded into common culture flask.The retronectin group was divided ...