| 增强miR-146b-3p表达对胰腺癌细胞增殖和凋亡的影响 |
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| 引用本文： | 林凡,王欣,俞亚红,王敏,洪晓泉.增强miR-146b-3p表达对胰腺癌细胞增殖和凋亡的影响[J].华中科技大学学报(医学版),2011,40(6). |
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| 作者姓名： | 林凡 王欣 俞亚红 王敏 洪晓泉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院胆胰外科,武汉,430030 |
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| 基金项目： | 国家自然科学基金，国家自然科学基金青年基金，湖北省卫生厅青年科技人才基金 |
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| 摘 要： | 目的 探讨增强miR-146b-3p表达对胰腺癌细胞增殖和凋亡的影响.方法 Real time PCR检测miR-146b-3p在6种人胰腺癌细胞系(MIA PaCa-2、BxPC-3、AsPC-1、PANC-1、SW1990、PC-3)和手术切除的12对胰腺癌/癌旁组织中的表达.将Pre-miR-hsa-miR-146b-3p Precursor 转染MIA PaCa-2 细胞,Real time PCR检测转染后miR-146b-3p的表达变化,四甲基偶氮唑蓝(MTT)法检测细胞增殖能力,流式细胞仪检测细胞凋亡率,免疫印迹法(Western blot)检测凋亡相关蛋白Bcl-2、Bax、Caspase-3、Caspase-8的表达变化.结果 与正常胰腺组织相比,miR-146b-3p在6种胰腺癌细胞系中均呈低表达,以MIA PaCa-2细胞最为明显;12对胰腺组织中,癌组织miR-146b-3p表达水平均低于癌旁组织.同阴性对照组比较,转染前体组的MIA PaCa-2细胞,miR-146b-3p表达增强383.25倍,细胞增殖能力下降,细胞凋亡率增加,蛋白Bcl-2表达降低(50.0%),Bax表达升高(4.29倍).结论 miR-146b-3p在胰腺癌细胞系和癌组织中低表达;上调胰腺癌细胞MIA PaCa-2中 miR-146b-3p的表达,能够抑制增殖和诱导细胞凋亡,其机制可能与下调Bcl-2表达,上调Bax的表达有关.
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| 关 键 词： | 胰腺癌 miR-146b-3p 细胞增殖 细胞凋亡 |
Effects of Up-regulated Expression of miR-146b-3p on Proliferation and Apoptosis of Pancreatic Cancer Cell Lines |
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| Lin Fan,Wang Xin,Yu Yahong et al.Effects of Up-regulated Expression of miR-146b-3p on Proliferation and Apoptosis of Pancreatic Cancer Cell Lines[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(6). |
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| Authors: | Lin Fan Wang Xin Yu Yahong |
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| Institution: | Lin Fan,Wang Xin,Yu Yahong et al Department of Biliary-Pancreatic Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the effects of the up-regulated miR-146b-3p on proliferation and apoptosis of pancreatic cancer cells.Methods The expression of miR-146b-3p in 6 pancreatic cancer cell lines and 12 matched pancreatic cancer and adjacent non-tumorous tissues were detected by real time PCR.Pre-miR-hsa-miR-146b-3p precursor was transfected into MIA PaCa-2 cells with transfection agent.The miR-146b-3p expression was detected by real time PCR.The cellular growth activity was assayed by MTT assay,and the ... |
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| Keywords: | pancreatic cancer miR-146b-3p cell proliferation cell apoptosis |
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