| 8-羟基二氢小檗碱对NIT-1细胞胰岛素分泌和葡萄糖代谢的影响 |
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| 引用本文： | 徐丽君,陆付耳,王增四,易屏,魏世超,董慧,邹欣.8-羟基二氢小檗碱对NIT-1细胞胰岛素分泌和葡萄糖代谢的影响[J].华中科技大学学报(医学版),2011,40(6):702-705. |
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| 作者姓名： | 徐丽君 陆付耳 王增四 易屏 魏世超 董慧 邹欣 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院,中西医结合研究所,武汉,430030
2. 华中科技大学同济医学院附属同济医院,药学部,武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的探讨8-羟基二氢小檗碱对NIT-1细胞胰岛素分泌的影响及其机制。方法采用不同浓度8-羟基二氢小檗碱干预NIT-1细胞后,以放射免疫法、液体闪烁计数法、酶法,分别检测其胰岛素水平、葡萄糖利用、葡萄糖激酶(GK)活性。结果在低浓度葡萄糖(5.5mmol/L)刺激下,与空白对照组相比,加入高浓度8-羟基二氢小檗碱(1.0μmol/L)的NIT-1细胞胰岛素分泌增加、葡萄糖利用活跃,且GK酶活性增强(P<0.05);在高浓度葡萄糖(16.5mmol/L)刺激下,与空白对照组相比,加入不同浓度8-羟基二氢小檗碱(0.5、1.0μmol/L)的NIT-1细胞胰岛素分泌增加、葡萄糖利用活跃,且GK酶活性增强(P<0.01或P<0.05)。结论 8-羟基二氢小檗碱能促进葡萄糖刺激下NIT-1细胞的胰岛素分泌,其机制可能与增加NIT-1细胞葡萄糖利用、增强GK酶活性有关。
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| 关 键 词： | 8-羟基二氢小檗碱 胰岛素 葡萄糖 葡萄糖激酶 NIT-1细胞 |
Effect of 8-hydroxy Dihydroberberine on Insulin Secretion and Glucose Metabolism in NIT-1 Cells |
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| Xu Lijun,Lu Fuer,Wang Zengsi et al Institute of Integrative Traditional , Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan ,China.Effect of 8-hydroxy Dihydroberberine on Insulin Secretion and Glucose Metabolism in NIT-1 Cells[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(6):702-705. |
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| Authors: | Xu Lijun Lu Fuer Wang Zengsi Institute of Integrative Traditional Western Medicine Tongji Hospital Tongji Medical College Huazhong University of Science Technology Wuhan China |
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| Institution: | Xu Lijun,Lu Fuer,Wang Zengsi et al Institute of Integrative Traditional and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To investigate the effect of 8-hydroxy dihydroberberine on insulin secretion in NIT-1 cells stimulated by glucose and the possible mechanism.Methods Radioimmunoassay,scintillation counting technique and enzymatic method were used to measure the effects of 8-hydroxy dihydroberberine on insulin secretion,glucose utilization and the activity of glucokinase(GK).Results As compared with untreated group,insulin secretion level,glucose utilization and the activity of GK in NIT-1 cells stimulated with low... |
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| Keywords: | 8-hydroxy dihydroberberine insulin glucose glucokinase NIT-1 cell |
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