抗HBsAg和抗红细胞双特异Diabody的构建及表达

目的构建及表达抗乙肝病毒表面抗原（ＨＢｓＡｇ）和抗红细胞（ＲＢＣ）双特异Ｄｉａｂｏｄｙ，用于血清中ＨＢｓＡｇ的快速检测。方法将抗ＨＢｓＡｇ的人抗体轻重链可变区基因与抗红细胞的鼠抗体重轻链可变区基因交叉配对构建成两个杂合Ｄｉａｂｏｄｙ基因，并将两个配对基因组建在一个表达载体中，成为双特异Ｄｉａｂｏｄｙ表达载体，并在大肠杆菌中分泌表达。结果经ＥＬＩＳＡ检测和红细胞凝集实验测定，证明所表达的双特异性Ｄｉａｂｏｄｙ具有抗ＨＢｓＡｇ和抗ＲＢＣ双重功能，并可使含有ＨＢｓＡｇ的ＲＢＣ悬液产生血球凝集。结论所表达的双特异性Ｄｉａｂｏｄｙ具有双重抗体活性，可用于血清中ＨＢｓＡｇ的快速检测

Construction and expression of anti HBsAg and anti RBC bispecific diabody.

Objective Construction and expression of anti HBsAg and anti RBC bispecific diabody for rapid detection of serum HBsAg. Methods The V L and V H genes of the anti HBsAg and anti RBC antibodies were first interchanged to form two hybrid diabody genes. Then the two hybrid diabody genes were combined together to form a bispecific diabody expression vector. The bispecific diabody was expressed in E.coli . Results The bispecific diabody was proved to be able to bind both HBsAg and RBC. It could cause agglutination of human RBC when HBsAg was present. Conclusions The bispecific diabody has the potential in rapid detection of serum HBsAg.