不同方法定量检测HIV／AIDS病例病毒载量分析

目的比较2种方法检测人类免疫缺陷病毒（HIV）1型病毒载量的相关性及一致性，为合理评价HIV／AIDS病程进展及抗HIV药物疗效提供参考。方法对四川省凉山州95例在治HIV／AIDS的血浆样本同时用分支DNA杂交实验（bDNA）法和核酸序列扩增实验（NASBA）法检测HIV-1病毒载量，结果进行比较分析。结果该2种方法测得阳性样本均值bDNA法为3．75±1．05logcopies／mL，NASBA法为4．22±1．23logcopies／mL，差异有统计学意义（t=6．09，P〈0．05）；2种方法测出的病毒载量呈显著直线相关（r=0．852，P〈0．01）；2种方法测出的病毒载量阳性率差异无统计学意义（x2=0．836，P〉0．05）。结论bDNA法和NASBA法检测HIV-1病毒载量具有高度的一致性，在实际工作中均可选用，但是两者检测结果的数值不能完全等同。建议抗HIV药物疗效评价最好应用同一种方法的检测结果，使其治疗前后的结果具有可比性和一致性。

Detection of HIV/AIDS Viral Loads With Two Different Quantitative Methods

Objective To compare the relativity and consistency between two different testing methods for quantitative detection of HIV - 1 plasma viral load, in order to rationally assess the progress of HIV/AIDS and the effieacy of anti - I＇tlV drugs. Methods Blood samples of 95 cages of HIV iecton an MDS patients re tested for HIV - 1 viral load by bDA and NASBA. Results The average HIV viral load dt13ted by bDNA and NAS- BA was 3.75 ＋ 1.05 g copies/mL 9/）d 4.22 ＋ 1.23 log copies/mL respectively, and tho difference was a statistically significant betwe-en tl tw.o groups t -： 09, P 〈 0. 05 ）. There was a aignificant linear correla- tion between the two methods （r = 0.001 ）： The difference showed no statistically significant between the positive rates of the two methods （X 2 =0.836 P〉0. 05）. Conclusion There is a high degree coincidence between the two methods for detecting HIV viral load,yet they can not be equally used in the prac- tical work. It is more preferable to employ only one method to evaluat the efficacy of anti - HIV drugs for a a better comparability and consistncy before and after treatment.