| 中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究 |
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| 引用本文： | 程春林,范雁,吴士良,邵一鸣,冯毅,何翔,林鹏,梁淑家,易志强,贺健梅,胡园园,邢辉. 中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究[J]. 中华流行病学杂志, 2003, 30(1): 720-725. DOI: 10.3760/cma.j.issn.0254-6450.2009.07.019 |
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| 作者姓名： | 程春林 范雁 吴士良 邵一鸣 冯毅 何翔 林鹏 梁淑家 易志强 贺健梅 胡园园 邢辉 |
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| 作者单位： | 中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制同家重点实验审,北京,100050;苏州大学医学部生物化学教研室;广东省疾病预防控制国心;广西壮族自治区疾病预防控制中心;江西省疾病预防控制中心;湖南省疾病预防控制中心;中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制同家重点实验审,胡园园,北京,100050; |
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| 基金项目： | 国家高技术研究发展计划"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项课胚 |
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| 摘 要： | Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.
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| 关 键 词： | 病毒株,HIV-1 流行簇 进化分析 |
Genetic characteristics of HIV-1 CRF01-AE strains in four provinces, southern China |
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| Keywords: | CRF01-AEHIV-1 CRF01_AE strainsEpidemic clusterPhylogenetic analysis |
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