The protection of creatine kinase MM sub-bands by EDTA during storage |
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Authors: | Jean-Paul Chapelle Alain Bertrand Camille Heusghem |
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Institution: | Laboratory of Clinical Chemistry, University of Liège, Rue des Bonnes- Villes, 1, B-4020 LiègeBelgium |
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Abstract: | We examined the effect of a 5 mmol/1 concentration of EDTA on the stabilization of the five serum creatine kinase MM isoenzymes, resolved by thin-layer isoelectric focusing.In patient sera, total CK and CK-MB activities were stable during storage of the samples for two months at 4° C even in the absence of EDTA. However, EDTA stabilized the labile MM and MM1 sub-bands, which are the first to appear in the blood after the release from the damaged tissue and its addition to blood samples intended for determining the MM sub-band pattern is recommended.The stabilizing effect of EDTA was emphasized at higher temperatures. EDTA protected the CK-MM pattern in myocardium extracts made in normal serum and incubated at 37°C during 40 h, but was unnecessary when myocardium was homogenized in heat-inactivated serum. It is thought that EDTA could act by inhibiting a heat-labile component of human serum. |
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Keywords: | To whom correspondence should be addressed |
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