Isolation and identification of a major urinary canthaxanthin metabolite in rats. |
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| Authors: | J Bausch H Liechti G Oesterhelt A Kistler |
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| Affiliation: | F. Hoffmann-La Roche Ltd., Basel, Switzerland. |
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| Abstract: | The urinary metabolic pattern after administration of the radiolabeled non-provitamin A carotenoid canthaxanthin was investigated in rats. In the rather complex HPLC urinary metabolic pattern a fraction was found which was conjugated. Deconjugation of the polar conjugates with glusulase, purification of the metabolite with HPLC and identification with GC-MS and NMR revealed that it was 3-hydroxy-4-oxo-7,8-dihydro-beta-ionone. This structure was confirmed by comparisons with HPLC retention times, UV/VIS- and NMR-spectroscopy and GC-MS of the synthesized compound. |
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