| 大蒜生理活性物质对胃癌细胞和黑色素瘤细胞抑制作用的研究 |
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| 引用本文： | 王霞,苟萍,孙桂琳,孙素荣,索菲娅.大蒜生理活性物质对胃癌细胞和黑色素瘤细胞抑制作用的研究[J].中成药,2010,32(4):557-561. |
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| 作者姓名： | 王霞 苟萍 孙桂琳 孙素荣 索菲娅 |
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| 作者单位： | 新疆大学生命科学与技术学院;新疆生物资源基因工程重点实验室;新疆大学; |
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| 基金项目： | 新疆生物资源基因工程重点实验室开放项目(XJDX0201-2009-08) |
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| 摘 要： | 目的:研究大蒜生理活性物质对人胃癌细胞MGC-9和小鼠黑色素瘤细胞B16细胞增殖及细胞周期的影响。方法:用倒置显微镜观察细胞形态,噻唑蓝(MTT)比色法测定细胞增殖抑制率;流式细胞仪检测B16细胞周期分布的影响。结果:经大蒜素及其前体物质蒜氨酸、蒜氨酸酶和蒜氨酸+蒜氨酸酶处理后,MGC-9和B16细胞不同程度变圆,体积缩小,脱壁细胞增多;除蒜氨酸酶外,蒜氨酸、大蒜素和蒜氨酸+蒜氨酸酶都能诱导MGC-9和B16产生时间和剂量依赖性的增殖抑制作用,其中蒜氨酸+蒜氨酸酶的作用尤为显著。大蒜素和蒜氨酸+蒜氨酸酶阻滞B16细胞于G0/G1期。结论:本文由大蒜提取物处理后结果与已发表的临床研究相一致。
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| 关 键 词： | 大蒜 大蒜素 蒜氨酸 蒜氨酸酶 人胃癌细胞MGC-9 小鼠黑色素瘤细胞B16 细胞增殖 细胞周期 |
Inhibitory effect of physiological active substances from garlic on gastric cancer cell and melanoma cell |
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| WANG Xia,GOU Ping,SUN Gui-lin,SUN Su-rong,SUO Fei-ya.Inhibitory effect of physiological active substances from garlic on gastric cancer cell and melanoma cell[J].Chinese Traditional Patent Medicine,2010,32(4):557-561. |
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| Authors: | WANG Xia GOU Ping SUN Gui-lin SUN Su-rong SUO Fei-ya |
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| Institution: | WANG Xia1,GOU Ping1,SUN Gui-lin1,SUN Su-rong1,2,SUO Fei-ya1(1.College of Life Science , Technology,Xinjiang University,Urumqi,Xinjiang 830046,China,2.Xinjiang Key Laboratory of Biological Resources ,Genetic Engineering of Ministry of Education,China) |
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| Abstract: | AIM: To study the effect of garlic extracts on cell proliferation and cell cycle on human gastric cancer cell line(MGC-9) and mouse murine melanoma cell line(B16).METHODS: Cell morphology was ob-served by inverse microscopy.Cytotoxicity was examined by dimethyl thiazolyl tetrazolium bromide assay.Cell cycle distribution was detected by flow cytometry.RESULTS: After being treated with allicin and its precursors-alliin,alliinase and mixture of alliin and alliinase from garlic extracts,cells rounded up,shrank ... |
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| Keywords: | garlic alliin alliicin alliinase human gastric cancer cell line MGC-9 mouse melanoma cell line B16 cell proliferation cell cycle |
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