胃癌患者肿瘤组织和外周血中细胞毒性T细胞的相关性分析

目的研究胃癌患者肿瘤浸润淋巴细胞CD8^＋CD28^＋细胞及CD8^＋CD28^-细胞数量与外周血CD8^＋CD28^＋细胞及CD8^＋CD28^-细胞数量的相关性。方法采用流式细胞术对60例胃癌患者的肿瘤浸润淋巴细胞及外周血淋巴细胞的CD8^＋CD28^＋细胞及CD8^＋CD28^-细胞进行检测，采用直线相关分析两者的相关性。结果胃癌患者外周血淋巴细胞CD8^＋CD28^-、CD8^＋CD28^＋细胞分别为（26．13±8．17）％和（8．15±4．00）％；肿瘤组织中肿瘤浸润淋巴细胞CD8^＋CD28^-、CD8^＋CD28^＋细胞分别为（36．98±15．30）％、（6．02±4．37）％。两者之间具有正相关性（r分别为0．602、0．387，P〈0．01）。结论胃癌患者外周血CD8^＋CD28^-、CD8^＋CD28^＋细胞的检测对了解患者的免疫状况具有一定的价值。

Analysis of the relation of the expression of cytotoxic T lymphocyte on peripheral blood lymphoeytes and neoplasm tissue in patients with gastric cancer

Objective To investigate the relation of the expression of CD8^＋ CD28^＋ and CD8^＋CD28 ^- in peripheral blood lymphoeytes to those of tumor infiltration lymphocyte in patients with gastric cancer. Methods Subjects for this investigation included peripheral blood cells and tumor infiltration lymphocyte from 60 patients with gastric cancer. The levels of the expression of CD8, CD28 T lymphocytes were analyzed by flow cytometry. The correlation was analyzed by linear correlation analysis. Results The level of the expression of CD8^＋ CD28^- and CD8^＋ CD28^＋ on peripheral blood lymphocytes in the patients with gastric cancer was（26.13±8.17）% and （8.15± 4.00）% respectively, however the level of those expression on tumor infiltration lymphocyte was （ 36.98±15.30 ） % and （ 6.02±4.37 ） % respectively. And these results were positive correlation （r=0. 602,0.387,P〈0.01）. Conclusions Detecting the expression of CD8^＋ CD28^＋ and CD8^＋ CD28^- in peripheral blood lymphocytes is valuable to find out the immune status of gastric cancer patients.