| 大鼠即刻早期基因产物Cyr61短发夹环 RNA质粒的构建与鉴定 |
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| 引用本文： | 付鹏,牛铁生,孙英贤. 大鼠即刻早期基因产物Cyr61短发夹环 RNA质粒的构建与鉴定[J]. 中国现代医学杂志, 2007, 17(22): 2714-2716 |
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| 作者姓名： | 付鹏 牛铁生 孙英贤 |
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| 作者单位： | 中国医科大学附属盛京医院,心血管内科,辽宁,沈阳,110004 |
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| 摘 要： | 目的构建Cyr61短发夹环RNA(shRNA),探讨即刻早期基因产物Cyr61RNAi对Cyr61功能的影响,以期为进行这方面研究提供基础。方法在Gene Bank中查到鼠Cyr61的mRNA基因序列并输入到相应设计软件中,以此设计引物序列。经PCR扩增、酶切后,克隆于Pgenesil-1载体并行酶切鉴定。结果构建的鼠即刻早期基因产物Cyr61shRNA质粒经测序鉴定验证与预期相符。结论该研究结果为研究Cyr61shRNA质粒的RNAi功能及其对Cyr61功能影响的研究奠定了基础。
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| 关 键 词： | RNA干扰 短发夹环RNA |
| 文章编号： | 1005-8982（2007）22-2714-03 |
| 收稿时间： | 2007-02-25 |
| 修稿时间： | 2007-02-25 |
Construction and identification of plasmid containing short hairp in RNA of Cyr61 of rats |
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| FU Peng,NIU Tie-sheng,SUN Ying-xian. Construction and identification of plasmid containing short hairp in RNA of Cyr61 of rats[J]. China Journal of Modern Medicine, 2007, 17(22): 2714-2716 |
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| Authors: | FU Peng NIU Tie-sheng SUN Ying-xian |
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| Abstract: | [Objective] To investigate the function of RNA interference and the plasmids containing short hairp in RNA of an angiogenic immediate early gene-cysteine-rich 61(Cyr61). [Methods] The sequence of rat Cyr61 mRNA was found in the Gene Bank. The primer was designed. The fragments amplificated with PCR were inserted to the Pgenesil-1 vector, and were selected and identified. [Results] The plasmid was certified to be in the right rank. [Conclusion] This study provided foundation for further research on the function of short hairp in RNA of Cyr61 and its effect on Cyr61. |
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| Keywords: | Cyr61 |
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