反复自然流产患者NO与Th1/Th2型细胞因子水平的变化

目的　探讨NO与Th1/Th2型细胞因子的水平在反复自然流产发病中的作用及相互关系。方法　应用硝酸还原酶法测定 3 0例非孕妇女、97例正常妊娠妇女、3 0例人工流产组、10 1例反复自然流产患者血清及胎盘中NO的含量。同时采用酶联免疫吸附试验测定血清中TNF、IL 6、IL 10的水平变化。结果　反复自然流产患者TNF、IL 6、NO的含量明显高于正常对照组 (P <0 0 5 >,IL 10的水平明显低于正常对照组 (P <0 0 1>,且NO的含量与IL 10呈负相关 (r =- 0 5 2 ,P <0 0 5 >,而与TNF的含量呈正相关 (r =0 72 ,P <0 0 5 )。结论　正常水平的NO维持正常妊娠的生理变化 ,含量过高可导致反复自然流产 ;Th1/Th2型细胞因子与反复自然流产有关 ,两型因子间的平衡受多种因素的调控 ,其中NO在调节Th1/Th2型细胞因子的平衡中起重要的作用

Study of relationship between nitric oxide and TH1/TH2 cytokines in patients with recurrent spontaneous abortion

Objective: To study the role and relationship of nitric oxide with TH1/TH2 cytokines in patient with recurrent spontaneous abortion (RSA). Methods: Serum and placenta NO were measured by nitrate reductase in 101 RSA and 30 nonpregnant women, 97 normal pregnant women, and 30 artificial abortion served as the control group. At the sametime ,serum TNF, IL-6,IL-10 were determined by enzyme linked immunosorbant assay (ELISA). Results: TNF, IL-6,NO in RSA groups were obviously higher than those in the normal control group (P<0.05),IL-10 in RSA was markedly lower than that in the control group (P<0.01).The concentration of NO in RSA was positively correlated with TNF (r=0.72,P<0.05), and negatively correlated with IL-10(r=-0.52,P<0.05). Conclusion: Normal level of NO maintain physiological changes in normal pregnant women. It may cause recurrent spontaneous abortion if NO content is very high. Th1/Th2 cytokines are related to recurrent spontaneous abortion. Two types of cytokines are regulated by various facts, of which NO plays an important role in regulating the balance of Th1/Th2 cytokines.