犬急性心房颤动时心房环氧酶-2的变化

目的：研究犬急性心房颤动（房颤）时心房内皮细胞环氧酶-2（COX-2）的变化。方法：12条健康成年杂种犬被随机分为快速心房起搏（RAP）组（6只）与正常对照组（6只），PAP组建立犬急性房颤动物模型。分别取两组犬的左心耳、右心耳部位心肌组织，应用免疫组织化学技术检测心耳内皮细胞COX-2的改变。结果：（1）RAP组所有犬均可诱发出阵发性房颤；（2）RAP组犬心耳内皮细胞COX-2阳性率为83．3％（10／12），对照组为25．0％（3／12），RAP组显著高于对照组（P=0．013）。结论：急性房颤时心耳内皮细胞COX-2水平增加，提示在房颤早期发生了炎症反应。

Change of level of cyclooxygenase-2 in atrium of canines with acute atrial fibrillation

Objective： To investigate the expression of eyclooxygenase-2 （COX-2） in right and left atrial appendages in acute atrial fibrillation （AF） model of caine. Methods： Twelve adult mongrel canines were randomly divided into rapid atrial pacing （RAP） group （n=6） and sham control group （control group, n=6）. A model of acute AF in RAP group has been developed. Immunohistochemistry was performed to detect the change of COX-2 at the protein level in endothelium of atrial appendages. Results： （1） Paroxysmal AF was induced in all of the canines of RAP group. （2） Immunohistochemistry study indicated that in RAP and control groups, the rate of COX--2 expression was 83.3 （10/12） and 25.0% （3/12） respectively. COX--2 expression was significantly stronger in the former （P=0. 013）. Conclusion： Acute AF in canines led to COX-2 protein expressed in RAP group showed its inflammation mechanism.