| 新型光敏剂CDHS801光动力学治疗T24细胞膀胱癌动物模型作用研究 |
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| 引用本文： | 宋海波,姜宁,王国增,贺伟,郑景存.新型光敏剂CDHS801光动力学治疗T24细胞膀胱癌动物模型作用研究[J].齐鲁药事,2009,28(8):493-496. |
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| 作者姓名： | 宋海波 姜宁 王国增 贺伟 郑景存 |
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| 作者单位： | 1. 山东省医药工业研究所,山东,济南,250100
2. 上海浦东新区公利医院,上海,200135 |
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| 摘 要： | 目的研究光敏剂CDHS801体内光动力治疗膀胱癌机理。方法T24细胞悬液裸鼠皮下注射制备膀胱癌裸鼠动物模型后,灌胃给予CDHS801,12h后激光照射肿瘤后处死动物,取瘤体进行HE染色、Tahel染色及电镜观察。结果激光照射后6h,肿瘤表面皮肤水肿,浅表血管扩张充血。HE染色发现肿瘤血管充血、血管形成血栓、大片区域肿瘤细胞变形坏死,TUNEL染色发现肿瘤细胞核内出现棕黄色颗粒,电镜提示细胞核内染色质浓集、边集于核膜下并出现新月体样变化,伴有线粒体肿胀、膜破裂、嵴断裂或消失等现象。结论CHDS801光动力学体内治疗膀胱癌,诱导肿瘤发生凋亡是其杀灭肿瘤的主要机理。
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| 关 键 词： | 光动力学治疗 光敏剂CDHS801 膀胱癌 体内研究 凋亡 |
Effect of new photosensitizer CDHS801-mediated photodynamic therapy on bladder cancer T24 cell xenograft model |
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| SONG Hai-bo,JIANG Ning,WANG Guo-zeng,HE Wei,ZHENG Jing-cun.Effect of new photosensitizer CDHS801-mediated photodynamic therapy on bladder cancer T24 cell xenograft model[J].qilu pharmaceutical affairs,2009,28(8):493-496. |
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| Authors: | SONG Hai-bo JIANG Ning WANG Guo-zeng HE Wei ZHENG Jing-cun |
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| Institution: | SONG Hai-bo,JIANG Ning1,WANG Guo-zeng1,HE Wei1,ZHENG Jing-cun1 |
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| Abstract: | OBJECTIVE To investigate the killing mechanism of CDHS801-based photodynamic therapy on the bladder cancer T24 cell xenograft models.METHODS Nude mice injected subcutaneously with T24 cells and the animal model of bladder carcinoma was successfully established.After CDHS801-based photodynamic therapy,changes of morphology and ultra-structure of the tumor tissues were investigated by HE staining,TUNEL and electron microscope therapy.RESULTS The bladder tumors of the transplanted nude mice of the PDT group sh... |
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| Keywords: | Photodynamic therapy photosensitizer CDHS801 bladder cancer research in vivoi apoptosis |
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