BAG1和TIMP3在结肠癌中的表达及临床病理意义

目的]探讨BAG1和TIMP3在结肠癌中的表达、两者相关性及临床病理意义。方法]采用免疫组织化学SABC法检测80例结肠癌组织和20例正常结肠黏膜巾蛋白BAG1、TIMP3的表达。结果]BAG1在结肠癌组织中阳性率（80％）显著高于正常组织的阳性率（10％）（P〈0.05）；TIMP3在结肠癌组织中的阳性率43.75％,而在正常黏膜组织的阳性率为60％（P〉0.05）。BAG1蛋白和TIMP3的表达与结肠癌分化程度、Duke’s分期、淋巴结转移及生存率密切相关（P〈0.05），与性别、年龄无关（P〉0.05）。蛋白BAGI与TIMP3表达无相关性（P〉0.051。结论]蛋白BAG1高表达和TIMP3表达缺失对结肠癌的发生发展均起重要作用,两者表达水平与结肠癌的浸润转移及恶性程度有关。BAG1和TIMP3可作为预测结肠癌浸润转移潜能的新的生物学指标。

Expressions of BAG1 and TIMP3 in Colon Carcinoma and Clinicopathologic Significance

Purpose] To explore the expression of BAG1 and tissue inhibitor of metalloproteinase 3 （TIMP3） in colon carcinoma and their correlation and clinicopathologic significance. Methods] SABC immunohistochemistry was used to detect the expression of BAG1 and TIMP3 in 80 colon car- cinoma tissues and 20 normal colonic mucosa. Results] Positive expression rate of BAG1 in colon carcinoma tissue （80%） was significantly higher than that in normal colonic mucosa （10%） （P〈0.05）. Positive expression rate of TIMP3 in colon carcinoma tissue （43.75%） wasn＇t significantly higher than that in normal colonic mucosa （60%） （P〉0.05）. Expression of BAG1 and TIMP3 was significantly correlated with colon carcinoma differentiation, Duke＇s staging, lymph node metastasis and survival rate （P〈0.05）, but not associated with gender and age （P〉0.05）. Moreover, BAG1 expression was not correlated with TIMP3. Conclusion] Over-expression of BAG1 or attenuated expression of TIMP3 may play an important role in genesis and development of colon carcinoma. The protein expression levels of BAGI and TIMP3 are related to the malignant degree, invasion and metastasis of colon carcinoma. BAG1 and TIMP3 might be new biological parameters in predicting invasion and metastasis of colon carcinoma.