两种冷冻载体对人胚胎玻璃化冷冻效果的影响

目的探讨不同载体对人早期胚胎玻璃化冷冻复苏的效果。方法采用半封闭式载体冷冻复苏胚胎的患者共265个周期，采用封闭式载体冷冻复苏胚胎的患者共136个周期，均采用玻璃化冷冻办法冷冻体外精-胚胎移植第3日的可利用胚胎，观察胚胎复苏和临床妊娠情况。结果半封闭式麦管冷冻复苏胚胎的患者移植周期258个，取消周期7个；复苏率、临床妊娠率和种植率分别为96．88％、48．44％和32．20％。封闭式麦管冷冻复苏胚胎的患者移植周期132个，取消周期4个；复苏率、临床妊娠率和种植率分别为95．31％、47．72％和32．28％。、两组患者的平均年龄、不孕年限、不孕类型、不孕原因、体外受精或卵胞质内单精子注射（IVF／ICSI）构成比以及胚胎复苏率、移植胚胎数、临床妊娠率和种植率比较差异均无统计学意义（P〉0．05）。结论 封闭式冷冻载体能获得与半封闭式载体同样的冷冻效果，同时能避免胚胎受病原微生物的交叉污染，是冷冻人早期胚胎的理想方法。

Comparison of two cryopreservation carriers in vitrification cryopreservation of human embryo

Objective To explore the effects of different cryopreservation carriers on vitrification cryopreservation of human embryos. Methods Human embryos of D3 were vitrification cryopreserved using different cryopreservation carriers （265 half-closing cryopreservation carriers and 136 closing cryopreservation carriers）. Pregnant outcomes were compared between the two groups. Results The survival rate, the clinical pregnancy rate, and the implantation rate of 258 （7 cancelled） half-closing cryopreservation carriers were 96. 88%, 48. 44%, and 32.20%, respectively. Whereas those rates of 132 （4 cancelled） closing cryopreservation carriers were 95.31% , 47. 72% , and 32.28% . The differences of mean age, years, types and causes of infertility, ratio of in vitro fetilization or intracytoplasmic sperm injection （IVF/ICSI）, embryo recovery rate and transfer number, rate of pregnancy, and implant rate between two groups were not statistically significant （P 〉 0. 05）. Conclusion The closing cryopreservation carrier was as effective as the half-closing cryopreservation carrier and can protect embryos from crossing contamination of pathogens. It is an ideal method to preserve human embryos.