Radioprotection of Tendon Tissue via Crosslinking and Free Radical Scavenging |
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Authors: | Aaron Seto Jr" target="_blank">Charles J GattJr Michael G Dunn |
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Institution: | (1) Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, UMDNJ-Robert Wood Johnson Medical School, 51 French Street, Orthopaedic Surgery, Room 424, PO Box 19, New Brunswick, NJ 08903, USA |
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Abstract: | Ionizing radiation could supplement tissue bank screening to further reduce the probability of diseases transmitted by allografts
if denaturation effects can be minimized. It is important, however, such sterilization procedures be nondetrimental to tissues.
We compared crosslinking and free radical scavenging potential methods to accomplish this task in tendon tissue. In addition,
two forms of ionizing irradiation, gamma and electron beam (e-beam), were also compared. Crosslinkers included 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide (EDC) and glucose, which were used to add exogenous crosslinks to collagen. Free radical scavengers included
mannitol, ascorbate, and riboflavin. Radioprotective effects were assessed through tensile testing and collagenase resistance
testing after irradiation at 25 kGy and 50 kGy. Gamma and e-beam irradiation produced similar degenerative effects. Crosslinkers
had the highest strength at 50 kGy, EDC treated tendons had 54% and 49% higher strength than untreated, for gamma and e-beam
irradiation respectively. Free radical scavengers showed protective effects up to 25 kGy, especially for ascorbate and riboflavin.
Crosslinked samples had higher resistance to collagenase and over a wider dose range than scavenger-treated. Of the options
studied, the data suggest EDC precrosslinking or glucose treatment provides the best maintenance of native tendon properties
after exposure to ionizing irradiation.
One or more of the authors (MGD) have received funding from the Musculoskeletal Transplant Foundation Peer Reviewed Scientific
Grants Program (January–December 2005). |
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