| 水蛭素抑制体外模拟增生性瘢痕模型的实验研究 |
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| 引用本文： | 李旋,刘达恩,张国佑,席庆春,牛占国,贾宇博,易成刚,夏炜,郭树忠. 水蛭素抑制体外模拟增生性瘢痕模型的实验研究[J]. 中华中医药杂志, 2009, 0(7) |
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| 作者姓名： | 李旋 刘达恩 张国佑 席庆春 牛占国 贾宇博 易成刚 夏炜 郭树忠 |
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| 作者单位： | 广西医科大学第一附属医院;合肥市第二人民医院;第四军医大学西京医院; |
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| 基金项目： | 广西医疗卫生重点科研课题(No.200314) |
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| 摘 要： | 目的:研究水蛭素对体外人增生性瘢痕模型的收缩能力,成纤维细胞的增殖、胶原分泌等功能的影响。方法:体外培养人增生性瘢痕成纤维细胞(HSFB),建立含增生性瘢痕成纤维细胞的胶原网架系统(FPCL),用1、10、50U/ml的水蛭素溶液干预该模型,测定凝胶块收缩指数,利用MTT染色计数活细胞,逆转录多聚酶链式反应(RT-PCR)法检测细胞Ⅰ、Ⅲ型前胶原mRNA表达。结果:浓度为10U/ml和50U/ml水蛭素溶液能够抑制HSFB的活力,使FPCL收缩指数降低,抑制FPCL中HSFBⅠ、Ⅲ型前胶原mRNA的表达(P<0.05)。结论:水蛭素溶液可抑制体外人增生性瘢痕三维模型的收缩功能和成纤维细胞增殖、分泌胶原功能。
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| 关 键 词： | 水蛭素 增生性瘢痕 成纤维细胞 三维培养 |
Experimental study on inhibition of human hypertrophic scar model in vitro by Hirudin |
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| LI Xuan,,LIU Da-en,ZHANG Guo-you,XI Qing-chun,NIU Zhan-guo,JIA Yu-bo,YI Cheng-gang,XIA Wei,GUO Shu-zhong. Experimental study on inhibition of human hypertrophic scar model in vitro by Hirudin[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2009, 0(7) |
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| Authors: | LI Xuan LIU Da-en ZHANG Guo-you XI Qing-chun NIU Zhan-guo JIA Yu-bo YI Cheng-gang XIA Wei GUO Shu-zhong |
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| Affiliation: | LI Xuan1,2,LIU Da-en1,ZHANG Guo-you1,XI Qing-chun2,NIU Zhan-guo1,JIA Yu-bo1,YI Cheng-gang3,XIA Wei3,GUO Shu-zhong3(1Department of Burn , Plastic Surgery,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China,2Plastic Surgery,The Second People's Hospital of Hefei,Hefei 230011,3Center of Plastic Surgery,Xijing Hospital,Fourth Military University of Chinese PLA,Xi'an 710032,China) |
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| Abstract: | Objective:To study the effect of Hirudin on contractibility of hypertrophic scar model,fibroblasts' proliferation and secretion in vitro.Methods:Human hypertrophic scar fibroblasts(HSFB) were cultured in vitro,and fibroblast populated collagen lattice(FPCL) was set up.The effect of Hirudin on HSFB model with exposure to 0U/ml,1U/ml,10U/ml or 50U/ml Hirudin for 24,48 or 72 hours in vitro were studied.We detected the cells' vitality and proliferation by MTT method,measured the gels' contractile index and asse... |
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| Keywords: | Hirudin Hypertrophic scar Fibroblast Fibroblast populated collagen lattice |
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