应用改良TA-Fe法显示肾的微血管及细胞的尝试

目的：光镜下观察改良TA—Fe法显示肾微血管和组织细胞的效果。方法：经TA—Fe法媒染的大鼠肾的两组冰冻切片，一组切片用改良方法经脱水、透明、封片，另一组切片用原TA—Fe法作为对照。结果：改良前后TA—Fe法均能显示肾微血管和肾小管。封存片后结构更清晰，肾血管球毛细血管分叶更明显。肾小囊上皮、毛细血管内皮细胞、足细胞以及球内系膜细胞核亦被良好显示。切片可较长时间保存，但立体感较改进前略差。结论：改良TA—Fe法显示肾微血管和组织细胞效果更好，适合做回顾性研究。

ATTEMPT OF DEMONSTRATING RENAL MICROVASCULATURE AND RENAL CELLS BY IMPROVED TA-FE METHOD

Objective:To observe the effect of staining renal microvasculature and renal cells by original tannic acid ferric chloride method and improved TA Fe method under light microscopy.Methods:The sections were made from the kidney then divided them into two groups,the sections from first group were dehydrated,cleared and mounted,another for original TA Fe staining.Finally all of those sections were photomicrographed.Results:The renal microvasculature and renal cells could be manifested distinctly by the both methods.Compared to the original TA Fe method,mounted sections is more distinguishable,more obvious in demonstrating renal microvasculature and renal cells.Lobes of glomerulus were more clearly visible.It is feasible to distinguish the cell nucleus from the Simple squamous epithelum of renal capsule,endothelium of capillaries,podocyte and intraglomerular mesangical cell.But the view in three dimensions is not very good.Conclusion:It is proved that improved TA Fe method is more practical in investigating the renal microvasculature and renal cells,more suitable for retrospective studies.