芦荟提取物C诱生小鼠肿瘤坏死因子的研究

目的 :研究芦荟提取物C(aloeextractC ,AE C)在BALB C鼠体内诱导产生肿瘤坏死因子 (tu mornecrosisfactor ,TNF)。方法 :连续 5d给小鼠注射AE C ,第 6天取血分离血清 ,应用结晶紫染色法测其TNF活性 ,观察经AE C诱生的鼠血清对多种瘤细胞的杀伤作用 ,杀伤作用的持续时间和对温度的敏感性及血清不同稀释度的杀伤效果。结果 :AE C能诱导小鼠产生TNF α ,与对照组比较作用明显 (P <0 0 1) ;并且对多种靶细胞有显著的杀伤作用 ;TNF活性 2 4h达高峰 ,第 4天下降明显 ,5d接近正常水平 ;血清标本稀释至 2 0 0倍时 ,仍具有较强的杀伤活性 ;诱生后的TNF有一定的温度敏感性。结论 :芦荟提取物C有诱生BALB C鼠产生肿瘤坏死因子的作用

Study on Aloe Extract C Induced TNF in Mice

Objective To study the effect on Aloe Extract C (AE C) inducing TNF in mice.Methods Mice were injected with AE C into abdominal cavity for 5 days,obtaining blood and separating serums in the sixth day.Y 99 ,L 929 and A 549 tumor cell lines were used as target cells to assay TNF activity in the serums,the activity in sensitizing for temperature and concentration of the serums.Results AE C induced TNF in BALB/C mice,and as compared with control it had a significant difference ( P <0 01).TNF activity reached a peak in 24 hours after injecting AE C and began to drop in the fouth day.The sample of serums had not activity after 5 days in room temperature and had still activity when it be diluted by 200 times.Conclusion AE C has the effect by inducing TNF in BALB/C mice. China J Cancer Prev Treat,2003,10(3):247-249