海桐种子醇提物中挥发性成分的GC-MS分析及抗内毒素活性测定

目的：研究海桐种子乙醇总提取物(简称EESPTA)的挥发性成分及相对含量,考察其抗内毒素活性。方法：以水蒸气蒸馏法提取EESPTA的挥发性成分,用气-质联用技术分析鉴定,并用峰面积归一化法测定各成分相对含量,采用终点基质显色法鲎实验检测其对细菌内毒素的清除效果,考察其抗内毒素活性。结果：气-质联用技术共检测鉴定出32个色谱峰,占该挥发性成分总量的99.82% 。在EESPTA的挥发性成分中,1R,4S,7S,11R-2,2,4,8-四甲基三环[5.3.1.0(4,11)]十一碳-8-烯含量高达54.78%,其它含量较高的成分有2-亚甲基-胆甾烷-3-醇(7.27%)、蛇麻烷-1,6-二烯-3-醇(6.23 %)、4-甲基-5-癸醇(4.11%)、绿花白千层醇(3. 86%)、别香橙烯(3. 80%)、反式-2,3-二甲基丙烯酸(3. 50%)、二氢-噻喃-3,5-二醇-四氢-4-甲基-4-氮-二乙酸乙酯(2. 04 %)等成分。结论：EESPTA具有较明显的清除细菌内毒素作用,并呈浓度依赖关系。为进一步开发利用海桐药用价值提供了科学依据。

GC-MS Analysis of Volatile Components in Ethanol Extract from Seeds of Pittosporum Tobira Ait. and Determination of Anti-endotoxin Activity

OBJECTIVE To analyze the volatile components in ethanol extract from seeds of Pittosporum tobira Ait.(EESPTA) and investigate the anti-endotoxin activity of EESPTA in vitro. METHODS The volatile components in EESPTA were extracted with steam distillation method and then analysized by GC-MS. The relative content of each component was calculated by peak area normalization. The anti-endotoxin activity of EESPTA in vitro was investigated by CEPTAL (chromogenic end-point tachypleus amebocyte lysate assay). RESULTS The 32 peaks were detected and then identified preliminary, which made up 99.82% of the total volatile components. The anti-endotoxin activity of EESPTA in vitro was observed in concentration-dependent manner from CEPTAL assay. The highest content constituent was 1R,4S,7S,11R-2,2,4,8-tetramethyltricyclo[5.3.1.0(4,11)] undec-8-ene (54.78%), and other relative high content constituents were 2-methylene-cholestan-3-ol(7.27%), humulane-1, 6-dien-3-ol(6.23%), 4-methyl-5-decanol(4.11%), viridiflorol(3.86%), Alloaromadendrene (3.80%), 2-methyl-2-butenoic acid(3.50%), et al. CONCLUSION EESPTA has obvious anti-endotoxin activity with a concentration dependence. This study provides a scientific basis for further development and utilization of Pittosporum tobira Ait..