荧光定量PCR法在结核杆菌检测中的价值

目的探讨荧光定量PCR法检测技术在结核病诊断中的价值。方法采用荧光定量PCR法和抗酸染色法同时检测231例结核患者体液标本(121例痰标本、67例胸腹水及43例尿液标本),对检测结果进行比较。结果荧光定量PCR法的检测灵敏度为102Copy/ml,是抗酸染色法方法检测灵敏度100倍,且对除结核杆菌外的其他呼吸道病原体检测结果均为阴性。本研究痰标本、胸腹水标本及尿液标本的荧光定量PCR法阳性率均要高于抗酸染色法阳性率,比较差异均有显著性(﹤0.01)。结论荧光定量PCR方法检测结核杆菌具有特异性强、灵敏度高、简便、快速的特点,明显优于抗酸染色法,可作为结核病诊断的常规方法。

Clinical value of fluorescence quantitative on detection of mycobacterium tuberculosis

Objective To investigate clinical value of fluorescence quantitative PCR on detection of mycobacterium tuberculosis. Methods 231 samples of body fluid (121 sputum samples,67 samples of pleural effusion and ascites and 43 urine samples) were detected by fluoresence quantitative PCR and acid-fast staining respectively. Sensitivity and specificity of fluoresence quantitative PCR were assessed by comparing testing results. Results Sensitivity of fluorescence quantitative PCR reached 102 Copy / ml, 100 times as high as that of acid-fasting staining. Furthermore, test results of other pathogens (mycoplasma, chlamydia, staphylococcus aureus, Pseudomonas aeruginosa) in respiratory tract were negative. Positive rate of fluoresence quantitative PCR in testing sputum samples,samples of pleural effusion and ascites and urine samples was higher that that of acid-fast staining, and there was a significant difference ( P<0.01).Conclusion Fluorescence quantitative PCR, characterized by distinct specificity, high sensitivity, simplicity and quick-response, is better than acid-fast staining and can be used as a conventional method to diagnose tuberculosis.