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| 高效液相色谱法测定葛瓜降糖胶囊中葛根素含量 | |
| 引用本文： | 孙法丽,马毅,王春香.高效液相色谱法测定葛瓜降糖胶囊中葛根素含量[J].中国药业,2007,16(20):33-34. |
| 作者姓名： | 孙法丽 马毅 王春香 |
| 作者单位： | 1. 山东省济南市中医院,山东,济南,250012 2. 山东轻工业学院,山东,济南,250100 3. 山东省莱芜市中医院,山东,莱芜,271100 |
| 摘 要： | 目的采用高效液相色谱（HPLC）法测定葛瓜降糖胶囊中葛根素的含量。方法色谱柱为Zorbax C18柱（250mm×4．6mm，5μm），流动相为甲醇-乙腈-水（8：10：82），流速为1．0mL／min，柱温为36℃，检测波长为250nm。结果葛根素进样量在0.201-4.020μg范围内与峰面积呈良好的线性关系，回归方程为y=35474．879X-101．1175（r=0．9999）；平均回收率为98．65％，RSD为0．99％（n=5）。结论HPLC法简便、准确、重现性好，能有效控制该制剂的质量。 |
| 关 键 词： | 高效液相色谱法 葛瓜降糖胶囊 葛根素 含量测定 |
| 文章编号： | 1006-4931（2007）20-0033-02 |
| 修稿时间： | 2007-02-26 |
Content Determination of Puerarin in Geguajiangtang Capsule by HPLC |
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| Sun Fali,Ma Yi,Wang Chunxiang.Content Determination of Puerarin in Geguajiangtang Capsule by HPLC[J].China Pharmaceuticals,2007,16(20):33-34. | |
| Authors: | Sun Fali Ma Yi Wang Chunxiang |
| Institution: | 1. Jinan Hospital of Traditional Chinese Medicine, Jinan, Shandong, China 250012; 2. Shandong College of light Industry, Jinan, Shandong, China 250100; 3. Laiwu Hospital of Traditional Chinese Medicine, Laiwu, Shandong, China 271100 |
| Abstract: | Objective To establish the method for determing Puerarin in Geguajiangtang Capsule by HPLC. Methods Zorbax C18 column was used with methanol-acetonitrile-water（8 ：10：82）as the mobile phase. The flow rate was 1 mL/min and the column temperature was 36℃. The content was detected at wavelength of 250 nm. Results The determination of Puerarin showed a good linear relationship in the range of 0.201 μg-4.020 μg（r=0.9999）.The average recovery rate was 98.65%, RSD was 0.99%. Conclusion The method is simple and accurate for the quality control of the Geguajiangtang Capsule. |
| Keywords: | HPLC Geguajiangtang Capsule puerarin content determination |
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