Reliability of Criteria for Ultrastructural Identification of Neuroendocrine Granules |
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Authors: | Irving Dardick Peter Rippstein Garth Perkins |
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Affiliation: | a Department of Pathology, Toronto Hospital, 200 Elizabeth Street, Toronto, Ontariob Department of Laboratory Medicine, Ottawa Civic Hospital, 1053 Carting Avenue, Ottawa, Ontario, Canada |
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Abstract: | For full diagnostic use to be made of the neurosecretory granule, the range of sizes, forms, and staining qualities for this cytoplasmic organelle, along with the extent of its expression in various neoplasms, must be established. Neurosecretory type granules occasionally occur in nonneuroendo-crine tumors. A series of carcinoids of the lung provides a model for assessing the morphologic types of cytoplasmic granules identified by antibodies to chromogranin A and immunogold labeling. The results show that granule structure in tumors is pleomorphic. Despite having sizes within the expected range, many labeled and, indeed, unlabeled secretory granules are atypical, particularly in structural form. Cell-to-cell variation in the proportion of even typical neurosecreotry granules labeling for chromogranin A is the rule. Studies correlating biochemical, immunohistochemical, electron microscopic, and perhaps in situ hybridization characteristics are required to define better the criteria for unequivocal identification of neurosecretory granules in tumors. |
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Keywords: | chromogranin lung tumors neuroendocrine secretory granule ultrastructure |
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