| NAG微量酶反应比色法在IL-2活性检测中的应用 |
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| 引用本文： | 郑杭民,杜平.NAG微量酶反应比色法在IL-2活性检测中的应用[J].免疫学杂志,1990(1). |
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| 作者姓名： | 郑杭民 杜平 |
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| 作者单位： | 第二军医大学微生物学教研室，第二军医大学微生物学教研室 上海 200433，上海 200433 |
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| 摘 要： | 本文研究了NAG微量酶反应比色法在IL-2活性检测中的应用,结果表明IL-2活性测定所用的CTLL-2细胞浓度与OD值呈直线关系(r=0.964715)。本文还探讨了NAG法在IL-2活性测定中CTLL-2细胞浓度,培养时间对检测效果的影响。本法与~3H—TdR掺入法和MTT法相比有较好的一致性。
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| 关 键 词： | IL—2活性测定 NAG |
Study of Application to Detection of IL-2 Activity by Using NAG Microcolorimetric Assay |
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| Zheng Hangmin Du Ping.Study of Application to Detection of IL-2 Activity by Using NAG Microcolorimetric Assay[J].Immunological Journal,1990(1). |
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| Authors: | Zheng Hangmin Du Ping |
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| Institution: | Zheng Hangmin Du Ping
Department of Microbiology,Second Military Medical University,Shanghai |
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| Abstract: | NAG has been used to develop a quantitation colorimetric assay for several celular biological assays. In this paper we successfully used the method to detect the activity of IL- 2 . By compared with the methods using 3H-TdR and MTT, we found the NAG method is as sensitive as other two methods. The free of radioisotope and precipitation of protien are theumain advantages of the colorimetric assay. |
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