游离细胞甘露糖受体的亲和细胞化学电镜观察

目的 对小鼠腹腔巨噬细腻及人精子的甘露糖受体（ＭＲ）进行超微结构定位。方法 采用甘露糖基化牛血清白蛋白（ＤＭＡ）结合１０ｎｍ胶体金制备探针ＤＭＡ－Ｇ１０,以此探针标记小腹腔巨噬细胞和获能前后的人精子,并用疼爱 射电子显微民制备的ＤＭＡ－Ｇ１０具有基露糖基结合特性。小鼠腹腔巨噬细胞可分为明、暗中类型。“暗细胞”不含ＭＲ,“明细胞”细胞膜表面含ＭＲ,该受体与ＤＭＡ－Ｇ１０结合后内化入细胞内。人 子发生

Affinity-Gold Cytochemistry Electronograph for Detection of Mannose Receptor of Free Cells

Objective\ To locate mannose recepter(MR) of mouse macrophage and human sperm on ultrastructural level.\ Methods\ D\|Mannosylated\|BSA(DMA,A8303) absorbed on colloidal gold particles of size 10 nm as a probe was used to detect MR of macrophage from mouse abdominal cavity and human sperm before or after capacitation, and then visualized by transmission electron microscopy.\ Results\ Complex probe of DMA\|G\-\{10\} can combined mannose residues specifically.\ Macrophages were divided into two subtypes, "light" and "dark".\ There was no receptor observed in "dark" macrophage.\ MRs distributed on the surface of plasma membrane of "light" macrophage and some entered the cells by endocytosis.\ In human sperm, MRs were labeled on the surface of inner acrosomal membrane or acrosomal vesicles of acrosome after capacitation in vitro, while in some sperm, the gold granules concentrated in a narrow band on the IAM of equatorial segment after acrosome reaction had completely finished.\ MRs were not detected in acrosome intact spermatozoa.\ Conclusion\ MR of mouse microphage was on plasma membrane,while MR of human sperm might be in acrosome.\ Affinity\|gold cytochemistry for detection of MR reported here was reliable and may be used on other cells study.