LYVE-1与血管内皮生长因子-C在喉鳞状细胞癌中的表达和意义

目的通过测定喉鳞状细胞癌组织内LYVE-1和血管内皮生长因子-C（VEGF-C）的表达情况，为喉鳞状细胞癌转移和预后的判定以及治疗提供一定的理论依据。方法通过免疫病理学方法检测LYVE-1的表达并计数喉鳞状细胞癌组织中淋巴管密度（LVD），RT-PCR法检测喉鳞状细胞癌中VEGF-C的表达，使用统计学方法对LVD和VEGF-C表达进行分析。结果喉癌组织内存在LYVE-1（＋）的管腔样结构，LYVE-1在喉癌组织和癌旁正常组织之间表达差异有统计学意义（P〈0．05），喉癌组织中VEGF-C mRNA的平均水平与正常组织之间差异有统计学意义（P〈0．05），瘤组织比正常组织高4-5倍，而且喉癌组织内VEGF-C表达与LVD之间存在相关性。结论喉鳞状细胞癌瘤组织中存在淋巴管；VEGF-C mRNA在喉鳞状细胞癌组织中的表达要比正常对照组织高，且VEGF-C mRNA的高表达可能会通过促进瘤内淋巴管的增生来促进喉癌的淋巴结转移。

Expression and clinical significance of LYVE-1 and VEGF-C in patients with laryngeal squamous cell carcinoma

Objective To elucidate the relationship between LYVE-1 and VEGF-C and their expression in laryngeal squamous cell carcinoma(LSCC),and provide theoretic evidence for the judgement of metastasis and prognosis of LSCC,also for the treatment.Methods An immunohistocbemical analysis was performed to 50 specimens of LSCC with lymphatic endothelial marker LYVE-1.Quantitation of lymphangiogenesis growth factor VEGF-C by RT- PCR was performed to 30 specimens of LSCC.Finally the correlation between LVD and VEGF-C mRNA was analyzed with statistics methods.Results LYVE-1 (+) was observed in all LSCC.The median copy number of VEGF-c mRNA was 4-5-fold higher in LSCC than in adjacent normal tissue.There was correlation between tumor VEGF-C mRNA copy number and intratumoral LVD.Conclusion Lymphatic vessels existed in LSCC.There was correlation between high levels of LVD in LSCC than in normal tissue.The high level of VEGF-C may accelerate the lymphatic metastasis by promoting the proliferation of lymphatic vessel.