| 左旋加替沙星的高效液相色谱测定方法的建立 |
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| 引用本文： | 曾佳,唐冰雯,周宏兵,杨泽民,杨爱群.左旋加替沙星的高效液相色谱测定方法的建立[J].解剖学研究,2009,31(6):453-454,461. |
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| 作者姓名： | 曾佳 唐冰雯 周宏兵 杨泽民 杨爱群 |
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| 作者单位： | 1. 广东药学院附属第一医院,广东,广州,510080
2. 广东药学院药物分析教研室,广东广州,510006 |
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| 基金项目： | 广东省医学科研基金项目 |
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| 摘 要： | 目的建立左旋加替沙星的HPLC测定方法。方法色谱柱:UhimateXB-C18柱(5μm,4.6 mm×250 mm);流速:1.0 mL/min;柱温:29℃;流动相:甲醇:0.08 mol/L磷酸二氢钾溶液为40:60,磷酸调节pH为3.87);检测波长:285nm;进样量:20μL。结果左旋加替沙星在浓度为10.3～51.5 μg/mL时线性关系良好,r=0.9995,平均回收率为98.0%;RSD为1.2%(n=6)。保留时间为9.887。结论该方法简单、准确,可作为开发左旋加替沙星制剂的质量控制。
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| 关 键 词： | 左旋加替沙星 高效液相色谱法 测定 |
Determine the contents of S-Gatifloxacin by HPLC |
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| ZENG Jia,TANG Bing-wen,ZHOU Hong-bing,YANG Ze-min,YANG Ai-qun.Determine the contents of S-Gatifloxacin by HPLC[J].Anatomy Research,2009,31(6):453-454,461. |
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| Authors: | ZENG Jia TANG Bing-wen ZHOU Hong-bing YANG Ze-min YANG Ai-qun |
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| Institution: | ZENG Jia,TANG Bing-wen,ZHOU Hong-bing,YANG Ze-min,YANG Ai-qun.The First Affiliated Hospital of Guangdong University of Pharmacy,Guangzhou 510080 China |
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| Abstract: | Objective To establish a HPLC method to determine the contents of S-Gatifloxacin.Methods The stationary phase was UltimateXB-C18 column; The flow rate was 1.0 mL/min and the temperature of column was 29℃.The mobile phase consisted of methanol-0.08mol/L KH_2PO_4 (40:60) adjusted to pH 3.87 with phosphoric acid.The detection wavelength was 285 nm.20 μL of sample solution was injected to the sampler.Results The calibration curve of S-Gatifloxacin was linear in the range of 10.3- 51.5 μg/mL(r=0.9995) with recovery of 98.0% and RSD of 1.2%(n=6).The retention time was 9.887.Conclusion The method is simple and accurate, and can be used in quality control of the development of S-Gatifloxacin preparation. |
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| Keywords: | S-Gatifloxacin HPLC Determine |
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