| 甘草次酸抑制K562细胞增殖的机制的实验研究 |
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| 引用本文： | 刘新月,杨海燕,陈开澜,孙春艳.甘草次酸抑制K562细胞增殖的机制的实验研究[J].中国医院药学杂志,2005,25(4):315-318. |
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| 作者姓名： | 刘新月 杨海燕 陈开澜 孙春艳 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院血研所,湖北,武汉,430022 |
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| 摘 要： | 目的:探讨甘草次酸(GA)抑制K562细胞增殖的机制.方法:对体外培养的K562细胞体系,采用MTT,放免,免疫组织化学的测定方法.结果:K562细胞抑制率与GA浓度及用药后培养时间呈正相关性.GA作用48 h后细胞上清液中血管紧张素Ⅱ(AngⅡ)含量明显上升.K562细胞中检测到AngⅡ 1型受体(AT1R)及2型受体(AT2R)蛋白的表达,但GA对其表达的影响无统计学意义.结论:GA可能通过抑制AngⅡ与AT1R的结合而抑制肿瘤细胞的增殖.
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| 关 键 词： | 甘草次酸 K562细胞 血管紧张素Ⅱ 血管紧张素Ⅱ 1型受体 血管紧张素Ⅱ 2型受体 |
| 文章编号： | 1001-5213(2005)04-0315-04 |
| 修稿时间： | 2004年12月22 |
Mechanism of inhibition of K562 leukemia cells proliferation by glycyrrhetinic acid |
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| LIU Xin-yue,YANG Hai-yan,CHEN Kai-lan,SUN Chun-yan.Mechanism of inhibition of K562 leukemia cells proliferation by glycyrrhetinic acid[J].Chinese Journal of Hospital Pharmacy,2005,25(4):315-318. |
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| Authors: | LIU Xin-yue YANG Hai-yan CHEN Kai-lan SUN Chun-yan |
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| Abstract: | OBJECTIVE To study the mechanism of inhibition of K562 leukemia cells proliferation by glycyrrhetinic acid (GA).METHODS The study was performed on cultured K562 leukemia cells by using MTT, radioimmunoassay,Immunohistochemical studies. RESULTS There was statistically significant difference in the inhibition rate and supernatant AngII level between the control group and GA groups. The expression of AngII type 1 receptor (AT_ 1 R) and AngII type 2 receptor (AT_ 2 R) were detected by Immunohistochemical studies,but the expression of them had no significantly difference between the control group and GA groups.CONCLUSION GA can inhibit the proliferation of K562 leukemia cells,potentially,through inhibition of the binding of AngII to its AT_ 1 R. |
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| Keywords: | glycyrrhetinic acid K562 leukemia cells angiotensin II AngII type 1 receptor AngII type 2 receptor |
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