Immunofluorescence and flow cytometry analysis of fibronectin and laminin binding to Sporothrix schenckii yeast cells and conidia |
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| Authors: | Lima Osana C Bouchara Jean-Philippe Renier Gilles Marot-Leblond Agnes Chabasse Dominique Lopes-Bezerra Leila M |
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| Affiliation: | 1. Department of Medicine, Division of Nephrology, Koc University School of Medicine, Istanbul, Turkey;2. Department of Medicine, Division of Nephrology, Konya Numune State Hospital, Konya, Turkey;3. Nephrology Clinic, Dialysis and Renal Transplant Center, ‘C.I. PARHON’ University Hospital, Romania;4. ‘Grigore T. Popa’ University of Medicine, Iasi, Romania;5. Department of Nephrology, Gülhane School of Medicine, Ankara, Turkey;6. Department of Radiology, Gülhane School of Medicine, Ankara, Turkey;7. Department of Biochemistry, Gülhane School of Medicine, Ankara, Turkey;8. Koc University School of Medicine, Ankara, Turkey;1. Clinical Research Unit, Dept. for Obstetrics & Gynecology, Technische Universitaet München, Munich, Germany;2. Institute for Advanced Study and Centre of Integrated Protein Science, Department Chemie, Technische Universitaet München, Garching, Germany;3. Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia;4. Dept. of Experimental Physics, University of Ulm, Ulm, Germany |
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| Abstract: | The adherence of Sporothrix schenckii yeast cells to several extracellular matrix (ECM) components has already been demonstrated, but the mechanisms of these interactions remained to be defined. In indirect immunofluorescence assays with polyclonal antibodies directed towards the ECM proteins, both hyphae and yeast cells of S. schenckii exhibited the ability to bind laminin and fibronectin. Flow cytometry confirmed the binding of these proteins, and revealed a significant greater binding capability for the yeast cells than for the conidia. Fibronectin and laminin binding was dose-dependent and specific. In addition, competition experiments with synthetic peptides mimicking the adhesive sequences of these proteins, or with cell wall fractions and carbohydrates constitutive of their sugar chains, were performed in order to specify the peptide or carbohydrate motifs involved in the recognition process. A 50% reduction was noticed in fibronectin binding in the presence of the synthetic peptide RGD, and a 38% reduction in laminin binding with the peptide YIGSR. Some carbohydrate-containing fractions of the yeast cell wall also inhibited the binding of fibronectin, but had no significant effect on laminin binding. Together, these results suggest the presence at the yeast surface of distinct receptors for laminin and fibronectin. |
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