突触素单克隆抗体免疫组化实验诊断肠神经发育不良症的价值

为探讨肠神经发育不良症（IND）的有效诊断方法，对45例术前诊断为先天性巨结肠（HD）的慢性肠梗阻患儿行巨结肠根治手术，并对其结肠痉挛段标本进行突触素单克隆抗体免疫组化实验。结果显示，16例结肠标本可见粘膜下神经丛过渡增生并有巨神经节（诊断为IND），与正常对照组比较，P＜0．01，其中14例有异位神经节（9例合并HD，下称HAIND），其病理切片中发现有异位的神经节细胞，且部分神经节细胞有变形，空泡化；10例IND（其中8例HAIND）可固有层和粘膜肌染色增强，与正常对照组比较，P＜0．01。认为突触素单克隆抗体免疫组化实验显示粘膜一神经丛过度增生，巨神经节可作为诊断IND的硬性指标；异位神经节，固有层和粘膜肌染色增强可作为诊断IND的辅助指标。在病理切片中应用突触素单克隆抗体免疫组化实验，可清晰显示肠壁神经丛的发育情况，是诊断IND的可靠方法。

Diagnostic value of synaptophisin to intestinal neuronal dysplasia

To observe the vaule of Monoclonal anti Synaptophsin in the diagnosis for intestinal neuronal dysplasia(IND),specimens from 45 patients with chronic constipation(suspected HD)after pull though operation were examined by immunohistochemistry method.Result showed that 16 patients appeared hyperganglionosis and giant ganglia in both the submucous and myenteric plexus,14(9HAIND)ectopic ganglion cells,and the activity in lamina propria and muscular mucosae were seen increased in 10 patients with IND(8 HAIND).Those suggest that the neurona plexus can be well seen by immunohistochemistry of Monoclonal anti Synaptophsin,this method is reliable in the diagnosis for IND.