| mtDNA SNPs研究方法进展及法医学应用现状 |
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| 引用本文： | 何琼,刘超,陈玲,王慧君.mtDNA SNPs研究方法进展及法医学应用现状[J].国际遗传学杂志,2010,33(1). |
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| 作者姓名： | 何琼 刘超 陈玲 王慧君 |
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| 作者单位： | 1. 南方医科大学法医学系,广州,510515
2. 广州市刑事科学技术研究所,510030 |
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| 基金项目： | 公安部重点科技攻关项目 |
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| 摘 要： | SNPs是不同个体基因组DNA序列中单个核苷酸的差异所致序列多态性,是继RFLP和STR标记发展起来的新一代遗传标记系统.线粒体是细胞内的惟一核外基因组,对于核DNA分型失败样本、降解样本、毛发(干)、指甲、骨骼等生物检材具有良好的实际应用价值.本文对目前线粒体SNPs分型最常用的技术及方法,如MALDI-TOF MS、SNaPshot~(TM)、焦磷酸测序、Illumina GoldenGate测序、Illumi-na Infinium测序、Taqman探针、SNPlex、SNPstream、分子信标技术及其在法医学实际应用进行了综述.
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| 关 键 词： | 线粒体DNA 法医学 |
Advances of mtDNA SNPs technologies and its application in forensic medicine |
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| Abstract: | Single nucleotide polymorphism (SNP) is defined as a single nucleotide difference giving rise to sequence polymorphism among individuals, as a new genetic marker following RFLP and microsatellite polymorphisms. Mitochondrial DNA (mtDNA) is a unique genomic DNA outside cell nucleus, and is very use-ful in the situation of analyzing failed samples of STR genotyping, degraded samples, hair (shafts), and finger nails in the long run. Here, new mtDNA SNP genotype technologies, including MALDI-TOF MS, SNaPshot~(TM) assay, Illumina GoldenGate assay, lllumina lnfinium assay, Taqman, SNPIex, SNPstream, molecular beacons, and their application in forensic medicine were reviewed. |
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| Keywords: | SNPs |
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