| 原位放射免疫分析在检测肺炎支原体克隆基因表达产物及临床微量标本上的应用 |
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| 引用本文： | 卢金,鲁润铭,郭慕华,陈庆学,王桂珍,李万军,董占双. 原位放射免疫分析在检测肺炎支原体克隆基因表达产物及临床微量标本上的应用[J]. 中国医科大学学报, 1993, 0(4) |
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| 作者姓名： | 卢金 鲁润铭 郭慕华 陈庆学 王桂珍 李万军 董占双 |
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| 作者单位： | 中国医科大学基础医学部微生物学教研室(卢金，鲁润铭，郭慕华，陈庆学，王桂珍，李万军)，中国医科大学基础医学部微生物学教研室(董占双) |
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| 摘 要： | 应用原位放射免疫分析法,从本实验室重组的以质粒为载体的重组子中,筛选出一株表达肺炎支原体抗原的克隆。同时应用此法对7例疑为非典型肺炎的患儿痰及咽嗽液标本进行检洲,其中4例出现阳性结果。试验表明此法也适用于临床微量抗原的直接检测。
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| 关 键 词： | 肺炎支原体 原位放射免疫分析 |
Radioimmunoassay in Situ Used to Detect Products of Coloning Mycoplasma Pneumoniae DNA and Clinical Microsamples |
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| Lu Jin,Lu Runming,Guo Muhua et al. Radioimmunoassay in Situ Used to Detect Products of Coloning Mycoplasma Pneumoniae DNA and Clinical Microsamples[J]. Journal of China Medical University, 1993, 0(4) |
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| Authors: | Lu Jin Lu Runming Guo Muhua et al |
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| Affiliation: | Dept. of Microbiology |
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| Abstract: | From the recombinants, which constructed in our laboratory, using plamids as vector, we obtained one strain which express antigen of Mycoplasma pneumoniae by detecting in situ radioimmunoassay. We also detected 7 children samples which were throat washing liquids of children suspected of atypical pneumoniae, 4 of them positive by using above method. This data indicated that it was suitable for clinical diagnosis directly. |
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| Keywords: | Mycoplasma pneumoniae radioimmunoassay in situ |
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