口腔乳杆菌对胶原的粘附

研究了包被在羟基磷灰石上的人Ｉ型胶原与＾３Ｈ－胸腺嘧啶脱氧核苷标记的乳杆菌的粘附能力及其影响因素。结果表明：乳杆菌对胶原有较高的亲和力，该粘附具有特异性，粘附量随人Ｉ型胶原浓度，粘附时间的增加而递增，并在２ｈ达到饱和。ｐＨ值对粘附有显著影响，ｐＨ值越低，粘附量越大，离子浓度对粘附也有明显影响。

Binding of Oral Lactobacilli to Collagen

The present study surveyed the ability, the characters and the influential factors on lactobacilli binding to the collagen. The experiment employed reference strains and fresh isolated strains. Binding of 3H-labeled bacteria to human type I collagen absorbed on hydroxyapatite surface (C-HA) was tested. The results suggested that the lactobacilli had high affinity for collagen. Competitive inhibitory assay indicated the attachment was highly specific. Binding to C-HA occurred in a collagen dose-dependent manner. pH value of reaction mixtures significantly affected the adherence. Below pH 6.5, increasing pH value caused a reduction of binding. Above pH 6.5, the binding was less affected. The ionic strength apparently influenced the binding level. The results of this study revealed one of the mechanisms that oral lactobacilli target dentin and root surfaces caries. This may also be a cariogenic virulent factor of lactobacilli.