The mechanism of actions of collagenase on the inhibition of release of acetylcholine from synaptosomal preparations. |
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| Authors: | G P Sgaragli I Sen A Baba R A Schulz J R Cooper |
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| Affiliation: | Department of Pharmacology, Yale University School of Medicine, New Haven, Conn. 06510, U.S.A. |
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| Abstract: | The release of acetylcholine from synaptosomal preparations from bovine superior cervical ganglia and rat cortex was inhibited when the preparations were pretreated with collagenase. The inhibition of release could be overcome with the calcium ionophore A23187. Collagenase treatment was shown to inhibit the uptake of calcium into the preparations. In addition, gel electrophoresis of synaptosomal membranes revealed two missing high molecular weight proteins when either synaptosomes or synaptosomal membranes were incubated with collagenase. |
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| Keywords: | To whom requests for reprints should be addressed. |
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