慢性重型乙肝病人血清sFas-sFasL复合体 ELISA检测方法的建立及应用

目的建立血清sFas-sFasL复合体ELISA检测方法,并探讨其在慢性重型乙肝病人发病机制研究中的意义.方法采用多抗体夹心ELISA法,检测血清sFas-sFasL复合体.检测了健康献血员37例、41例无症状已肝病毒携带者、40例慢性乙型重型肝炎及42例乙肝病毒相关性肝细胞癌患者血清sFas-sFasL复合体.结果慢性重型肝炎病人血清sFas-sFasL复合体阳性率显著高于健康对照组、无症状乙肝病毒携带者、乙肝相关性肝细胞肝癌组.结论本研究建立的ELISA方法可以较好地检测出血清中的sFas-sFasL复合体;慢性乙型重型肝炎患者血清中存在sFas-sFasL复合体.

Establish and Applification of the ELISA Method in Detection of sFas - sFasL Complements in Patients with Chronic Serious Hepatitis

Objective To establish a method to detect sFas - sFasL complements in serum, and study it's effect in the pathogenesis of chronic hepatitis B. Methods sFas - sFasL complements were detected with a new ELISA method in four groups,41 Asymptomatic HBsAg carriers , 40 cases of chronic serious hepatitis B and 42 cases of HBV related HCC, 37 healthy blood donor as control group. Results The positive rate of sFas - sFasL complements in chronic serious hepatitis B group was high significantly that of other 3 groups. Conclusion The ELISA method we established in this study can detect the sFas - sFasL compliments in serum. The sFas - sFasL compliments may play an important roles in hepatic apoptosis and necrosis,and may be applied as important clue in diagnosing pathogenetic condition and prognosis in chronic serious hepatitis B.