| 针刺督脉穴对血管性痴呆模型大鼠行为及细胞凋亡影响的实验研究 |
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| 引用本文： | 张冠楠,鄂建羽,殷晓莉.针刺督脉穴对血管性痴呆模型大鼠行为及细胞凋亡影响的实验研究[J].辽宁中医杂志,2011(8):1667-1670. |
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| 作者姓名： | 张冠楠 鄂建羽 殷晓莉 |
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| 作者单位： | 大连医科大学;辽宁中医药大学附属二院; |
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| 基金项目： | 辽宁省重点专科建设项目 |
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| 摘 要： | 目的:评价针刺督脉穴对血管性痴呆(Vascular dementia,VD)大鼠模型的学习记忆改善情况及可能机制,为针刺治疗血管性痴呆提供理论依据。方法:采用两侧颈总动脉血管阻断加硝普钠降压法复制VD大鼠模型;对VD大鼠模型进行西药(喜得镇)、针刺和电针干预;采用morris水迷宫法观察学习记忆情况;应用免疫组化法测定海马CA1区Bcl-2、Bax蛋白表达及原位末端标记法检测细胞凋亡。结果:(1)morris水迷宫学习记忆能力检测,经过6次训练,与对照组比较,模型组跳台时间延长;与模型组比较,各治疗组跳台时间明显缩短,差异均有统计学意义(P<0.05)。(2)凋亡相关蛋白检测结果显示:与对照组比较,模型组海马组织bcl-2、bax及细胞凋亡明显增高,差异均有统计学意义(P<0.01);与模型组比较,各治疗组海马组织bcl-2水平增高,bax及细胞凋亡水平明显降低,差异均有统计学意义(P<0.01)。结论:(1)各治疗组均可改善VD模型大鼠的认知能力,提高学习记忆成绩。(2)各治疗组均可抑制VD大鼠海马组织细胞凋亡。
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| 关 键 词： | 针刺 血管性痴呆(VD) 海马 氧化应激 细胞凋亡 |
Study on the Effect of Acusector on Behavior and Apoptosis of Hippocampi Tissue in rat |
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| ZHANG Guan-nan,ER Jian-yuadvisor:YIN Xiao-li.Study on the Effect of Acusector on Behavior and Apoptosis of Hippocampi Tissue in rat[J].Liaoning Journal of Traditional Chinese Medicine,2011(8):1667-1670. |
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| Authors: | ZHANG Guan-nan ER Jian-yuadvisor:YIN Xiao-li |
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| Institution: | ZHANG Guan-nan1,ER Jian-yu1advisor:YIN Xiao-li2(1.Dalian Medical University,Dalian 116044,Liaoning,China,2.the Second Hospital Attached to Liaoning University of TCM,Shenyang 110034,China) |
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| Abstract: | Objective:Evaluating the effect of acusector on Du Channel on improving memory and its mechanism,in order to provide theory basis in treatment Vascular dementia.Methods:Estabolishing Vascular dementia model by interdicting carotids in two sides combined with sodium nitroprusside.Treatment Vascular dementia with hydergine,acupuncture and acusector.Observed leaning memory by the method of water maze.Determined bcl-2 and Bax expression and apoptosis by immunohistochemistry and TUNEL respectively.Results:(1) Th... |
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| Keywords: | acusector Vascular dementia hippocampi apoptosis |
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