黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞毒性研究

目的：研究黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞的毒性。方法：75%乙醇回流提取得黄药子提取物，高、中、低剂量作用于Caco-2细胞，以经Caco-2细胞的摄取物作用于HL-7702细胞和HepG2细胞，进行细胞活性实验，测定生化指标ALT、AST、GSH-PX和MDA值。结果：与对照组比，高剂量组和中剂量组黄药子醇提物Caco-2细胞摄取液作用后，HL-7702细胞和HepG2细胞的存活率显著降低（P<0.01）；高剂量组黄药子醇提物Caco-2细胞摄取液作用72 h后，HL-7702细胞上清液中ALT、AST显著升高（P<0.01）；高剂量组黄药子醇提物Caco-2细胞摄取液作用48 h和72 h后， HepG2细胞上清液中ALT、AST显著升高（P<0.01）。高剂量组和中剂量组黄药子醇提物Caco-2细胞摄取液作用48 h和72 h后，HL-7702细胞和HepG2细胞上清液中MDA显著升高，GSH-PX显著降低（P<0.01）。结论：黄药子醇提物Caco-2细胞摄取液对HL-7702和HepG2细胞有毒性。

Toxicity Study of the Uptaking Compositions of Diosooroa Bulbifera L Alcohol Extract on HL-7702 and HepG2 Cell

OBJECTIVE To study the toxicity of the uptaking compositions of Diosooroa bulbifera L alcohol extract in HL-7702 and HepG2 cell. METHODS After reflux extraction with 75% alcohol, the alcohol extracts of Diosooroa bulbifera L at high, middle and low doses were added to Caco-2 cell model. Then the uptaking compositions were added to HL-7702 and HepG2 cell for the determination of the cytoactive and the biochemical indicator of ALT, AST, GSH-PX and MDA. RESULTS Compared with the control group, the high and middle dose groups decreased the survival rate of HL-7702 and HepG2 cell (P<0.01). The high dose group increased ALT and AST of HL-7702 cell supernatant after 72 h (P<0.01). The high dose group increased ALT and AST of HepG2 cell supernatant after 48 h and 72 h (P<0.01). MDA were significant increased and GSH-PX were significant decreased in the supernatant of HL-7702 and HepG2 cell at high and middle doses after 48 h and 72 h (P<0.01). CONCLUSION The result showed that the uptaking compositions of Diosooroa bulbifera L alcohol extract had toxic effect in HL-7702 and HepG2 cell.