| 铁皮枫斗HPLC特征图谱研究 |
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| 引用本文： | 刘东辉, 杨丽娥, 黄月纯, 魏刚. 铁皮枫斗HPLC特征图谱研究[J]. 中国现代应用药学, 2013, 30(4): 390-394. |
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| 作者姓名： | 刘东辉 杨丽娥 黄月纯 魏刚 |
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| 作者单位： | 1.广州中医药大学，广州510006;2.广州中医药大学第一附属医院，广州510405 |
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| 基金项目： | 广东省产学研合作项目项目(2009B090300333)；广州市番禺区科技计划项目(2010-专-12-11) |
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| 摘 要： | 目的 建立铁皮枫斗HPLC特征图谱。 方法 采用Zorbax SB C18色谱柱；流动相为乙腈-0.2%的甲酸溶液，梯度洗脱；检测波长为270 nm；柱温为35 ℃；流速为1.0 mL·min-1。 结果 铁皮枫斗标示出36个特征共有峰，10批样品的相似度为0.898~0.969。 结论 本方法准确可靠，重复性好，为铁皮枫斗的质量控制提供了一定的方法依据。
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| 关 键 词： | 铁皮枫斗 特征图谱 高效液相色谱法 |
| 收稿时间： | 2012-04-02 |
| 修稿时间： | 2013-01-22 |
Analysis of HPLC Characteristic Spectrum on Dendrobium Officinale of Fengdous |
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| LIU Donghui, YANG Li’e, HUANG Yuechun, WEI Gang. Analysis of HPLC Characteristic Spectrum on Dendrobium Officinale of Fengdous[J]. Chinese Journal of Modern Applied Pharmacy, 2013, 30(4): 390-394. |
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| Authors: | LIU Donghui YANG Li’e HUANG Yuechun WEI Gang |
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| Affiliation: | 1.Guangzhou University of TCM, Guangzhou 510006, China;2.The First Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510405, China |
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| Abstract: | OBJECTIVE To establish the method of characteristic spectrum analysis on Dendrobium officinale of Fengdous by HPLC. METHODS HPLC was used with the Zorbax SB C18 column, the acetonitrile-0.2%methanoic acid solution with gradient elution was employed as a mobile phase, detection wavelength was 270 nm, column temperature was 35 ℃, and flow rate was 1.0 mL·min-1. RESULTS Thirty six common peaks were separated in Dendrobium officinale of Fengdous, the similarities of 10 batches were 0.898-0.969. CONCLUSION The method is accurate and reliable, has a better reproducibility, and provides a reference for the quality control of Dendrobium officinale of Fengdous. |
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| Keywords: | Dendrobium officinale species of Fengdous characteristic spectrum HPLC |
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