HPLC测定中肺合剂中防己诺林碱和粉防己碱的含量

 目的测定中肺合剂中防己诺林碱、粉防己碱的含量。方法样品经预处理后采用RP-HPLC同时测定它们的含量。色谱条件:色谱柱为Agilent Zorbax Elipse XDB-C₁₈;流动相为0.06%三乙胺-乙腈溶液(44∶56);流速为1.0 mL·min^-1;检测波长设在240 nm;柱温30℃。结果防己诺林碱在0.437 5～17.5 mg·L^-1与粉防己碱在0.875～35.0 mg·L^-1内,峰面积与其浓度具有良好的线性关系。平均加样回收率分别为89.30%～94.35%(防己诺林碱),90.08%～95.74%(粉防己碱)。结论本方法简便、快速、准确,可同时测定中肺合剂中防己诺林碱和粉防己碱的含量。

Determination of Fangchinoline and Tetraridrine in Zhongfei Mixture by HPLC

OBJECTIVE To establish a method for the determination of fangchinoline and tetraridrine in Zhongfei mixture.METHODS The separation was performed on an Agilent Zorbax Elipse XDB-C₁₈ column at 30 ℃ and detected at 240 nm,the mobile phase consisted of 0.06% triethylamine and acetonitrile(44∶56) at a flow rate of 1.0 mL·min^-1.RESULTS The calibration curves of fangchinoline and tetraridrine had excellent linearity between the range of 0.437 5-17.5 mg·L^-1 and 0.875-35.0 mg·L^-1.The average recoveries of spiked with sample were 89.30%-94.35% for fangchinoline and 90.08%-95.74% for tetraridrine.CONCLUSION The method is sensitive,convenient and accurate,and is suitable for the simultaneous determination of fangchinoline and tetraridrine in Zhongfei mixture.