| ACTIVATION OF NUCLEOLAR rRNA GENE TRANSCRIPTION IN LYMPHOCYTES BY TUMOR PROMOTERS: APPLIES STUDIES WITH IN SITU HYBRIDIZATION DETECTED BY FLUORESCENT METHOD |
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| 引用本文： | 林仲翔,吕桂芝,周立新,韩亚玲,高燕,Franz Wachtler. ACTIVATION OF NUCLEOLAR rRNA GENE TRANSCRIPTION IN LYMPHOCYTES BY TUMOR PROMOTERS: APPLIES STUDIES WITH IN SITU HYBRIDIZATION DETECTED BY FLUORESCENT METHOD[J]. 中国癌症研究, 1992, 4(3): 1-9. DOI: 10.1007/BF02997210 |
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| 作者姓名： | 林仲翔 吕桂芝 周立新 韩亚玲 高燕 Franz Wachtler |
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| 作者单位： | Department of Cell Biology，Beijing Institute far Cancer Research，Department of Cell Biology，Beijing Institute far Cancer Research，Department of Cell Biology，Beijing Institute far Cancer Research，Department of Cell Biology，Beijing Institute far Cancer Research，Department of Cell Biology，Beijing Institute far Cancer Research，Histologische-Embryologisches der Universitat Wien，Austria |
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| 摘 要： | The distribution of rDNA was visualized in interphase nuclei of tumor promoter treated human lymphocytes in comparison with the mltogen Phytoheamagglutinin (PHA) effects by using an in situ hybridization fluorescent method. The procedure involves biotinylated rDNA as the probe and FITC-avidin as detection system. Silver (Ag) staining was used to visualize nucleoli. In the interphase nuclei of most of the nonstimulated control lymphocytes, only one small Ag-stained nucleolus could be seen. The in situ hybridization, however, revealed one to several agglomerations of rDNA fluorescent spots. With tumor promoting herb extract WCE (40 μg/ml) or TPA (60 ng/ ml) treatment, the Interphase nucleoli increased slightly in number with the morphology alteration into larger, reticular or compact granular types. Ag-stained particles also increased in number. The number of the in situ hybridization rDNA fluorescent spots and dots increased markedly and largereticulate formations of numerous rDNA spots were seen. This
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Activation of nucleolar rrna gene transcription in lymphocytes by tumor promoters: Applies studies with in situ hybridization detected by fluorescent method |
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| Zhongxiang Lin,Guizhi Lü,Lixin Zhou,Yaling Han,Yan Gao,Wachtler Franz. Activation of nucleolar rrna gene transcription in lymphocytes by tumor promoters: Applies studies with in situ hybridization detected by fluorescent method[J]. Chinese Journal of Cancer Research, 1992, 4(3): 1-9. DOI: 10.1007/BF02997210 |
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| Authors: | Zhongxiang Lin Guizhi Lü Lixin Zhou Yaling Han Yan Gao Wachtler Franz |
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| Affiliation: | Lin Zhongxiang Lu Guizhi Zhou LixinHan Yaling Gao YanDepartment of Cell Biology,Beijing Institute far Cancer ResearchFranz WachtlerHistologische-Embryologisches der Universitat Wien,Austria |
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| Abstract: | |
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| Keywords: | rDNA. in situ hybridization fluorescent method. Wikstroemla Chamaedaphne Extract (WCE). TPA. PHA. |
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