RP-HPLC内标法测定大鼠血浆中盐酸氟桂利嗪的含量

目的建立RP-HPLC内标法测定大鼠血浆中盐酸氟桂利嗪含量的方法。方法色谱柱：Shimadzu ODS C18柱（150mm×4.6mm,5μm）;流动相：甲醇-磷酸二氢钾（0.01mol/L,pH=3.5）（68：32）;流速：1.0mL/min;检测波长：254nm;柱温：30℃;进样量：20μL;内标：对羟基苯甲酸丙酯。结果盐酸氟桂利嗪在0.01～0.12μg/mL范围内线性关系良好,r=0.9997（n=7）,信噪比（S/N）〉3时,最低检测浓度为3.48×10-3μg/mL;平均回收率为89.83%,RSD值为2.76%。结论本方法简便、灵敏,回收率和精密度高,可用于盐酸氟桂利嗪新剂型研究中血药浓度的测定及其药代动力学研究。

Determination of flunarizine hydrochloride in rats plasma by RP-HPLC

Objective To establish an RP-HPLC internal standard method for the determination of flunarizine hydrochloride in rats plasma.Methods The analysis was performed on a Shimadzu ODS C18 column(150 mm×4.6 mm,5 μm);the mobile phase was methanol-potassium dihydrogen phosphate(0.01 mol/L,pH=3.5)(68:32) at a flow rate of 1.0 mL/min;the detection wavelength was 254 nm and the column temperature was 30 ℃;the injection volume was 20 μL and the internal standard was hydroxybenzoate.Results There was a good linear relationship of flunarizine hydrochloride in the range of 0.01-0.12 μg/mL,r = 0.999 7(n = 7).When the signal to noise ratio(S/N) > 3,the minimum detectable concentration was 3.48×10-3 μg/mL;the average recovery of the plasma samples was 89.83% and the RSD was 2.66%.Conclusion This method is both simple and sensitive with high recovery and precision,and can be used in plasma concentration determination and pharmacokinetic studies of flunarizine hydrochloride.