Selective enrichment in human megakaryocyte progenitors by the B203.13 surface differentiation antigen |
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| Authors: | Marco,Vitale ,Giorgio,Zauli ,Alessandra,Bassini ,Giuseppe,Visani ,Rita,Rezzani ,Carlo M.,Croce & Francesco Antonio, Manzoli |
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| Affiliation: | Department of Biomedical Sciences and Biotechnology, Human Anatomy Section, Brescia,;Institute of Human Anatomy, University of Ferrara, Ferrara,;Institute of Haematology,;Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, U.S.A.,;Institute of Human Anatomy, University of Bologna, Bologna, Italy |
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| Abstract: | The B203.13 monoclonal antibody specifically recognizes a subset (8–18.5%) of human CD34+ haemopoietic progenitors, which are significantly ( P < 0.05) enriched in megakaryocyte progenitors (CFU-meg). Moreover, B203.13 expression was progressively lost as maturation proceeded along the megakaryocytic lineage. In fact: (i) CFU-meg derived from CD34+/B203.13+ showed a greater number of cells/colony with respect to CD34+/B203.13−; (ii) after 10 d of liquid cultures with 100 ng/ml of thrombopoietin, only a minority of CD34-derived cells positive for CD41 coexpressed B203.13; (iii) in situ immunocytochemical staining revealed that B203.13 was expressed exclusively on immature megakaryoblasts; (iv) circulating platelets did not express B203.13. |
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| Keywords: | megakaryocytes platelets cell differentiation |
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