人树突状细胞体外提呈凋亡胆管癌细胞抗原的研究

目的：建立从人外周血诱导扩增树突状细胞（ＤＣ）及其从恨癌细胞提呈抗原的方法。方法：从正常人外周血分离获得单核细胞，加入５０ｎｇ／ｍｌ ｈＧＭ－ＣＳＦ，１０００ｎｇ／ｍｌ ｈＩＬ－４，隔天１次共４次，培养第３天，加入γ射线照射过和胆管癌细胞，再继续体外培养１周后，用树－突状细胞富集柱收集ＤＣ。结果：ＤＣ高表达共刺激分子Ｂ７和ＣＤ１ａ，表面具有典型不规则突起，ＤＣ捕捉凋亡小体、吞噬凋亡小体，负载抗原的

Acquirement of antigen from apoptotic cells by human dendritic cells from peripheral blood

Objective: To seek an efficient way of antigen acquirement from apoptotic cells by human dendritic cells (DC) from peripheral blood. Methods: We obtained DC from peripheral blood monocytes maintaining the antigen capturing and processing capacity by using granulocyte/macrophage colony stimulating factor (GM CSF) and interleukin 4 (IL 4). Then the immature DC and apoptotic hepatocholangioma cells were cocultured for 7 d. Results: These cells have typical dendritic morphology and express high levels of CD1a and B7. Furthermore, they acquire antigen from apoptotic cells and induce and increased T cell stimulatory capacity in MLR. Conclusion: We established DC from blood monocytes using GM CSF and IL 4. DC can efficiently acquire antigen derived from apoptotic cells and induce increase of T cells.