| 中药水蛭提取及精制工艺的研究 |
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| 引用本文： | 姚琳,王伟明,张妍妍,孙妍.中药水蛭提取及精制工艺的研究[J].中国实验方剂学杂志,2011,17(3):31-33. |
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| 作者姓名： | 姚琳 王伟明 张妍妍 孙妍 |
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| 作者单位： | 黑龙江省中医研究,哈尔滨,150036 |
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| 摘 要： | 目的:研究中药水蛭的提取及精制工艺,为水蛭提取物入制剂工艺提供技术依据。方法:依正交试验法,逆流循环提取技术,以效价法检测抗凝血酶活性为指标,对水蛭提取工艺进行优化,并运用膜分离技术对提取液进行精制。结果:最佳工艺为以12倍量0.9%氯化钠溶液提取5 h,循环3次最好,选择相对分子质量为30×103和0.5×103的膜即可达到精制的目的。结论:该提取、精制工艺稳定、合理、可行。
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| 关 键 词： | 水蛭 逆流循环提取 膜分离技术 精制 效价法 |
| 收稿时间： | 2010/9/13 0:00:00 |
Studies on Extraction and Purification Technology of Chinese Medicine Leeches |
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| YAO Lin,WANG Wei-ming,ZHANG Yan-yan and SUN Yan.Studies on Extraction and Purification Technology of Chinese Medicine Leeches[J].China Journal of Experimental Traditional Medical Formulae,2011,17(3):31-33. |
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| Authors: | YAO Lin WANG Wei-ming ZHANG Yan-yan and SUN Yan |
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| Institution: | YAO Lin,WANG Wei-ming,ZHANG Yan-yan,SUN Yan(Heilongjiang Academy of Traditional Chinese Medicine,Haerbin 150036,China) |
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| Abstract: | Objective:Studies on extraction and purification technology of the Chinese medicine leeches,preparing process of extracting leeches to provide technical basis.Method:The extraction of leeches was optimized by orthogonal experiment and countercurrent circuit extractive technique,with titer detected by anti-thrombin activi-ty as an index,and using of membrane separation technology to refine extract.Result: The best technology was to extract five hours with 12 times the amount of 0.9% sodium chloride solution,... |
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| Keywords: | leech countercurrent circuit extractive technique membrane separation technique purifica-tion determination of tobramycin by turbidimetric method |
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